Development of a proteoliposome model to probe transmembrane electron-transfer reactions

Gaye F. White; Zhi Shi; Liang Shi; Alice C. Dohnalkova; James K. Fredrickson; John M. Zachara; Julea N. Butt; David J. Richardson; Thomas A. Clarke

doi:10.1042/BST20120116

Development of a proteoliposome model to probe transmembrane electron-transfer reactions

Gaye F. White, Zhi Shi, Liang Shi, Alice C. Dohnalkova, James K. Fredrickson, John M. Zachara, Julea N. Butt, David J. Richardson, Thomas A. Clarke

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

11 Downloads (Pure)

Abstract

The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.

Original language	English
Pages (from-to)	1257-1260
Number of pages	4
Journal	Biochemical Society Transactions
Volume	40
Issue number	6
DOIs	https://doi.org/10.1042/BST20120116
Publication status	Published - 2012

Access to Document

10.1042/BST20120116

White_et_al_Biochem_Soc_Trans_2012_Development_of_a_model_for.pdfOther version, 247 KB

Cite this

@article{14216e33e4d94856a9558da23430fba4,

title = "Development of a proteoliposome model to probe transmembrane electron-transfer reactions",

abstract = "The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.",

author = "White, {Gaye F.} and Zhi Shi and Liang Shi and Dohnalkova, {Alice C.} and Fredrickson, {James K.} and Zachara, {John M.} and Butt, {Julea N.} and Richardson, {David J.} and Clarke, {Thomas A.}",

year = "2012",

doi = "10.1042/BST20120116",

language = "English",

volume = "40",

pages = "1257--1260",

journal = "Biochemical Society Transactions",

issn = "0300-5127",

publisher = "Portland Press Ltd.",

number = "6",

}

Development of a proteoliposome model to probe transmembrane electron-transfer reactions. / White, Gaye F.; Shi, Zhi; Shi, Liang; Dohnalkova, Alice C.; Fredrickson, James K.; Zachara, John M.; Butt, Julea N.; Richardson, David J.; Clarke, Thomas A.

In: Biochemical Society Transactions, Vol. 40, No. 6, 2012, p. 1257-1260.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development of a proteoliposome model to probe transmembrane electron-transfer reactions

AU - White, Gaye F.

AU - Shi, Zhi

AU - Shi, Liang

AU - Dohnalkova, Alice C.

AU - Fredrickson, James K.

AU - Zachara, John M.

AU - Butt, Julea N.

AU - Richardson, David J.

AU - Clarke, Thomas A.

PY - 2012

Y1 - 2012

N2 - The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.

AB - The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.

U2 - 10.1042/BST20120116

DO - 10.1042/BST20120116

M3 - Article

VL - 40

SP - 1257

EP - 1260

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

IS - 6

ER -