Development of a proteoliposome model to probe transmembrane electron-transfer reactions

Gaye F. White, Zhi Shi, Liang Shi, Alice C. Dohnalkova, James K. Fredrickson, John M. Zachara, Julea N. Butt, David J. Richardson, Thomas A. Clarke

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)
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Abstract

The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.
Original languageEnglish
Pages (from-to)1257-1260
Number of pages4
JournalBiochemical Society Transactions
Volume40
Issue number6
DOIs
Publication statusPublished - 2012

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