Diagnosing rotavirus A associated IID: Using ELISA to identify a cut-off for real time RT-PCR.

Gemma Phillips; Ben Lopman; Clarence C Tam; Miren Iturriza-Gomara; David Brown; J Gray

Diagnosing rotavirus A associated IID: Using ELISA to identify a cut-off for real time RT-PCR.

Gemma Phillips, Ben Lopman, Clarence C Tam, Miren Iturriza-Gomara, David Brown, J Gray

Norwich Medical School

Research output: Contribution to journal › Article

69 Citations (Scopus)

Abstract

ELISA is the best method for the laboratory diagnosis of rotavirus A associated IID. If RT-PCR is used, it is advisable to use a real time platform and to use a viral load cut-off equivalent to the detection limit of ELISA.

Original language	English
Pages (from-to)	242-245
Number of pages	4
Journal	Journal of Clinical Virology
Volume	44
Issue number	3
Publication status	Published - Mar 2009

Cite this

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title = "Diagnosing rotavirus A associated IID: Using ELISA to identify a cut-off for real time RT-PCR.",

abstract = "ELISA is the best method for the laboratory diagnosis of rotavirus A associated IID. If RT-PCR is used, it is advisable to use a real time platform and to use a viral load cut-off equivalent to the detection limit of ELISA.",

author = "Gemma Phillips and Ben Lopman and Tam, {Clarence C} and Miren Iturriza-Gomara and David Brown and J Gray",

year = "2009",

month = mar,

language = "English",

volume = "44",

pages = "242--245",

journal = "Journal of Clinical Virology",

issn = "1386-6532",

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AU - Phillips, Gemma

AU - Lopman, Ben

AU - Tam, Clarence C

AU - Iturriza-Gomara, Miren

AU - Brown, David

AU - Gray, J

PY - 2009/3

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N2 - ELISA is the best method for the laboratory diagnosis of rotavirus A associated IID. If RT-PCR is used, it is advisable to use a real time platform and to use a viral load cut-off equivalent to the detection limit of ELISA.

AB - ELISA is the best method for the laboratory diagnosis of rotavirus A associated IID. If RT-PCR is used, it is advisable to use a real time platform and to use a viral load cut-off equivalent to the detection limit of ELISA.

M3 - Article

VL - 44

SP - 242

EP - 245

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 3

ER -