Diagnostic and clinical significance of Crohn’s disease specific pancreatic anti-GP2 and anti-CUZD1 antibodies

Polychronos Pavlidis, Alastair Forbes, Dimitrios P Bogdanos

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Background: Pancreatic autoantibodies (PAB) targeting GP2 and CUZD1 are Crohn’s disease (CrD)-markers. The clinical significance of anti-GP2 antibodies has been assessed, but that of anti-CUZD1 remains elusive.
Aim: To assess the clinical utility of anti-CUZD1/anti-GP2 by novel cell-based indirect immunofluorescence (IIF) assays in CrD.
Methods: A total of 212 CrD and 249 UC patients followed-up at a London IBD centre were investigated to simultaneously detect PABs, anti-GP2 and anti-CUZD1 by IIF using primate pancreatic tissue, and HEK293 over-expressing CUZD1 or GP2.
Results: Overall, 88 (41.5%) CrDs compared to 26 (10.4%) UCs (p<0.001) tested positive for IgA and/or IgG anti-GP2 and/or anti-CUZD1 antibodies, while ASCA were found in 67.5% CrDs vs 19.2% UCs (p<0.0001); ASCA and/or PAB (anti-GP2 or anti-CUZD1) were detected in 76% CrD vs 34% UC patients. IgG anti-GP2 antibodies were less prevalent in L2 phenotype (p=0.002) and more prevalent in patients with stricturing disease (p=0.0418), even when a higher cut off (≥1,000 RU) was used (p=0.0396). Also, anti-GP2 IgG positive CrD patients had younger age of disease onset. IgA and/or IgG ASCA & anti-GP2 IgG antibody positive CrDs had younger onset of disease (p<0.0001), were more likely to have both ileal and colonic disease (p<0.0001) and had more stricturing (p <0.0001) than seronegative patients. Clinical correlates were not found for anti-CUZD1 positivity.
Conclusions: PAB testing increases ASCA’s serological sensitivity for CrD. Anti-GP2 detection, in isolation or in combination with ASCA, stratify CrD patients which phenotypically are characterised by a much younger onset of disease, extensive and stricturing behaviour.
Original languageEnglish
Pages (from-to)176–181
JournalClinical Chemistry and Laboratory Medicine
Volume441
Early online date12 Dec 2014
DOIs
Publication statusPublished - 20 Feb 2015

Keywords

  • Autoantibody
  • Bowel disease
  • Marker
  • Sensitivity
  • Specificity

Cite this