Dietary alpha-tocopherol affects differential gene expression in rat testes

Cristina Rota, Luca Barella, Anne Marie Minihane, Elisabeth Stöcklin, Gerald Rimbach

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Gene-chip technology was employed to study the effect of dietary vitamin E (VE) on gene expression in rat testes. Male albino rats were fed with either a diet deficient in VE or a standard diet containing VE. Differential gene expression was monitored at five individual time-points over a period of 14 months with all animals individually profiled. Low VE intake resulted in the consistent up-regulation of 7-dehydrocholesterol reductase and GATA binding protein 4, both involved in testosterone synthesis. Cyclin D3, important in cell cycle progression and Wilms tumor 1, related to cancer development, were also up-regulated in the vitamin E deficient animals. This study demonstrates that low dietary VE intake has long-term effects on gene expression in the testes. Our data provides insights into the possible molecular mechanisms underlying the beneficial effects of vitamin E on the male reproductive organ.
Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalIUBMB Life (International Union of Biochemistry and Molecular Biology: Life)
Issue number5
Publication statusPublished - May 2004

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