Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1

Freddy Boutrot; Cécile Segonzac; Katherine N. Chang; Hong Qiao; Joseph R. Ecker; Cyril Zipfel; John P. Rathjen

doi:10.1073/pnas.1003347107

Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1

Freddy Boutrot, Cécile Segonzac, Katherine N. Chang, Hong Qiao, Joseph R. Ecker, Cyril Zipfel, John P. Rathjen

Research output: Contribution to journal › Article › peer-review

181 Citations (Scopus)

Abstract

In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.

Original language	English
Pages (from-to)	14502-14507
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America (PNAS)
Volume	107
Issue number	32
DOIs	https://doi.org/10.1073/pnas.1003347107
Publication status	Published - 10 Aug 2010

Keywords

Arabidopsis
Arabidopsis Proteins
Ethylenes
Gene Expression Regulation, Plant
Immunity, Innate
Nuclear Proteins
Protein Kinases
Receptors, Immunologic
Transcription Factors

Access to Document

10.1073/pnas.1003347107

Cite this

Boutrot, F., Segonzac, C., Chang, K. N., Qiao, H., Ecker, J. R., Zipfel, C., & Rathjen, J. P. (2010). Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107(32), 14502-14507. https://doi.org/10.1073/pnas.1003347107

@article{703b19def7554110942d9d4f7113f89e,

title = "Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1",

abstract = "In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.",

keywords = "Arabidopsis, Arabidopsis Proteins, Ethylenes, Gene Expression Regulation, Plant, Immunity, Innate, Nuclear Proteins, Protein Kinases, Receptors, Immunologic, Transcription Factors",

author = "Freddy Boutrot and C{\'e}cile Segonzac and Chang, {Katherine N.} and Hong Qiao and Ecker, {Joseph R.} and Cyril Zipfel and Rathjen, {John P.}",

year = "2010",

month = aug,

day = "10",

doi = "10.1073/pnas.1003347107",

language = "English",

volume = "107",

pages = "14502--14507",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "32",

}

Boutrot, F, Segonzac, C, Chang, KN, Qiao, H, Ecker, JR, Zipfel, C & Rathjen, JP 2010, 'Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1', Proceedings of the National Academy of Sciences of the United States of America (PNAS), vol. 107, no. 32, pp. 14502-14507. https://doi.org/10.1073/pnas.1003347107

Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1. / Boutrot, Freddy; Segonzac, Cécile; Chang, Katherine N. et al.

In: Proceedings of the National Academy of Sciences of the United States of America (PNAS), Vol. 107, No. 32, 10.08.2010, p. 14502-14507.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1

AU - Boutrot, Freddy

AU - Segonzac, Cécile

AU - Chang, Katherine N.

AU - Qiao, Hong

AU - Ecker, Joseph R.

AU - Zipfel, Cyril

AU - Rathjen, John P.

PY - 2010/8/10

Y1 - 2010/8/10

N2 - In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.

AB - In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Ethylenes

KW - Gene Expression Regulation, Plant

KW - Immunity, Innate

KW - Nuclear Proteins

KW - Protein Kinases

KW - Receptors, Immunologic

KW - Transcription Factors

U2 - 10.1073/pnas.1003347107

DO - 10.1073/pnas.1003347107

M3 - Article

C2 - 20663954

VL - 107

SP - 14502

EP - 14507

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 32

ER -