Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation

Adam D. Celiz; James G.W. Smith; Asha K. Patel; Andrew L. Hook; Divya Rajamohan; Vinoj T. George; Luke Flatt; Minal J. Patel; Vidana C. Epa; Taranjit Singh; Robert Langer; Daniel G. Anderson; Nicholas D. Allen; David C. Hay; David A. Winkler; David A. Barrett; Martyn C. Davies; Lorraine E. Young; Chris Denning; Morgan R. Alexander

doi:10.1002/adma.201501351

Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation

Adam D. Celiz, James G.W. Smith, Asha K. Patel, Andrew L. Hook, Divya Rajamohan, Vinoj T. George, Luke Flatt, Minal J. Patel, Vidana C. Epa, Taranjit Singh, Robert Langer, Daniel G. Anderson, Nicholas D. Allen, David C. Hay, David A. Winkler, David A. Barrett, Martyn C. Davies, Lorraine E. Young, Chris Denning, Morgan R. Alexander

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Abstract

A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.

Original language	English
Pages (from-to)	4006-4012
Number of pages	7
Journal	Advanced Materials
Volume	27
Issue number	27
Early online date	1 Jun 2015
DOIs	https://doi.org/10.1002/adma.201501351
Publication status	Published - 15 Jul 2015

Keywords

high throughput
human pluripotent stem cells
materials discovery
polymer microarrays
stem cell differentiation

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10.1002/adma.201501351Licence: CC BY

Accepted_ManuscriptAccepted author manuscript, 832 KBLicence: CC BY
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http://eprints.nottingham.ac.uk/48601/Licence: CC BY

James Smith
- J.G.Smithuea.acuk
- Norwich Medical School - Associate Professor
- Metabolic Health - Member
- Cardiovascular and Metabolic Health - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching & Research

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Celiz, A. D., Smith, J. G. W., Patel, A. K., Hook, A. L., Rajamohan, D., George, V. T., Flatt, L., Patel, M. J., Epa, V. C., Singh, T., Langer, R., Anderson, D. G., Allen, N. D., Hay, D. C., Winkler, D. A., Barrett, D. A., Davies, M. C., Young, L. E., Denning, C., & Alexander, M. R. (2015). Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation. Advanced Materials, 27(27), 4006-4012. https://doi.org/10.1002/adma.201501351

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title = "Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation",

abstract = "A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.",

keywords = "high throughput, human pluripotent stem cells, materials discovery, polymer microarrays, stem cell differentiation",

author = "Celiz, {Adam D.} and Smith, {James G.W.} and Patel, {Asha K.} and Hook, {Andrew L.} and Divya Rajamohan and George, {Vinoj T.} and Luke Flatt and Patel, {Minal J.} and Epa, {Vidana C.} and Taranjit Singh and Robert Langer and Anderson, {Daniel G.} and Allen, {Nicholas D.} and Hay, {David C.} and Winkler, {David A.} and Barrett, {David A.} and Davies, {Martyn C.} and Young, {Lorraine E.} and Chris Denning and Alexander, {Morgan R.}",

year = "2015",

month = jul,

day = "15",

doi = "10.1002/adma.201501351",

language = "English",

volume = "27",

pages = "4006--4012",

journal = "Advanced Materials",

issn = "0935-9648",

publisher = "Wiley",

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Celiz, AD, Smith, JGW, Patel, AK, Hook, AL, Rajamohan, D, George, VT, Flatt, L, Patel, MJ, Epa, VC, Singh, T, Langer, R, Anderson, DG, Allen, ND, Hay, DC, Winkler, DA, Barrett, DA, Davies, MC, Young, LE, Denning, C & Alexander, MR 2015, 'Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation', Advanced Materials, vol. 27, no. 27, pp. 4006-4012. https://doi.org/10.1002/adma.201501351

TY - JOUR

T1 - Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation

AU - Celiz, Adam D.

AU - Smith, James G.W.

AU - Patel, Asha K.

AU - Hook, Andrew L.

AU - Rajamohan, Divya

AU - George, Vinoj T.

AU - Flatt, Luke

AU - Patel, Minal J.

AU - Epa, Vidana C.

AU - Singh, Taranjit

AU - Langer, Robert

AU - Anderson, Daniel G.

AU - Allen, Nicholas D.

AU - Hay, David C.

AU - Winkler, David A.

AU - Barrett, David A.

AU - Davies, Martyn C.

AU - Young, Lorraine E.

AU - Denning, Chris

AU - Alexander, Morgan R.

PY - 2015/7/15

Y1 - 2015/7/15

N2 - A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.

AB - A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.

KW - high throughput

KW - human pluripotent stem cells

KW - materials discovery

KW - polymer microarrays

KW - stem cell differentiation

U2 - 10.1002/adma.201501351

DO - 10.1002/adma.201501351

M3 - Article

VL - 27

SP - 4006

EP - 4012

JO - Advanced Materials

JF - Advanced Materials

SN - 0935-9648

IS - 27

ER -

Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation

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Keywords

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James Smith

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