Discrepancy between impedance and immunofluorescence platelet counting has implications for clinical decision making in patients with idiopathic thrombocytopenia purpura

Kristian M Bowles, David M Bloxham, David J Perry, Trevor P Baglin

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12 Citations (Scopus)


This study investigated whether differences occur between the impedance and immunofluorescence methods for platelet quantification in idiopathic thrombocytopenia purpura (ITP). Immunofluorescence gave a platelet count >50% higher than the impedance test in 9/35 (26%) patients, of which 4/35 (11%) were >100% higher. The clinical severity of thrombocytopenia was changed as a result of the immunofluorescence test in 14/35 (40%) patients. Neither mean platelet volume nor platelet distribution width predicted impedance/immunofluorescence method discrepancy. It is suggested that immunofluorescence platelet counts should be performed on all ITP patients when the implementation of a therapeutic or diagnostic intervention is being considered.
Original languageEnglish
Pages (from-to)320-2
Number of pages3
JournalBritish Journal of Haematology
Issue number3
Publication statusPublished - Aug 2006


  • Blood Coagulation
  • Fluorescent Antibody Technique
  • Humans
  • Patient Selection
  • Platelet Count
  • Purpura, Thrombocytopenic, Idiopathic

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