TY - JOUR
T1 - Distinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis
AU - Korycka-Machala, Malgorzata
AU - Brzostek, Anna
AU - Rozalska, Sylwia
AU - Rumijowska-Galewicz, Anna
AU - Dziedzic, Renata
AU - Bowater, Richard
AU - Dziadek, Jaroslaw
PY - 2006/5
Y1 - 2006/5
N2 - Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and in their response to chemicals that damage DNA. The results provide in vivo evidence that only NHEJ is required for the repair of noncompatible DNA ends. By contrast, the response of mycobacteria to mitomycin C preferentially involved a RecA-dependent pathway.
AB - Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and in their response to chemicals that damage DNA. The results provide in vivo evidence that only NHEJ is required for the repair of noncompatible DNA ends. By contrast, the response of mycobacteria to mitomycin C preferentially involved a RecA-dependent pathway.
U2 - 10.1111/j.1574-6968.2006.00199.x
DO - 10.1111/j.1574-6968.2006.00199.x
M3 - Article
SN - 1574-6968
VL - 258
SP - 83
EP - 91
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -