Distribution, proliferation, and function of Paneth cells in uncomplicated and complicated adult celiac disease

Antonio Di Sabatino; Emanuela Miceli; Winnie Dhaliwal; Paolo Biancheri; Raffaele Salerno; Laura Cantoro; Alessandro Vanoli; Massimo De Vincenzi; Camillo Del Vecchio Blanco; Thomas T MacDonald; Gino R Corazza

doi:10.1309/5ADNAR4VN11TTKQ6

Distribution, proliferation, and function of Paneth cells in uncomplicated and complicated adult celiac disease

Antonio Di Sabatino, Emanuela Miceli, Winnie Dhaliwal, Paolo Biancheri, Raffaele Salerno, Laura Cantoro, Alessandro Vanoli, Massimo De Vincenzi, Camillo Del Vecchio Blanco, Thomas T MacDonald, Gino R Corazza

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Paneth cells, granulated epithelial cells located at the base of small bowel crypts, have a crucial role in innate immunity. Because controversies remain concerning Paneth cell numbers and function in celiac disease (CD), we quantified Paneth cells and human alpha-defensin (HD)-5 and HD-6 in 28 patients with uncomplicated CD, 8 patients with complicated CD (3 with ulcerative jejunoileitis, 2 with refractory sprue, and 3 with enteropathy-associated T-cell lymphoma), and 14 control subjects. Paneth cell numbers and proliferation did not differ in uncomplicated untreated and treated CD and control cases. However, the number of Paneth cells was significantly reduced in complicated CD. Mucosal HD-5 and HD-6 were comparable in uncomplicated untreated and treated CD and control cases. Ex vivo gliadin challenge of treated CD biopsy specimens had no effect on mucosal HD-5 and HD-6 transcripts. Paneth cell numbers and alpha-defensins are unchanged in the mucosa in uncomplicated CD. Further studies are needed to clarify the implications of reduction of numbers of Paneth cells in complicated CD.

Original language	English
Pages (from-to)	34-42
Number of pages	9
Journal	American Journal of Clinical Pathology
Volume	130
Issue number	1
Early online date	7 Jan 2008
DOIs	https://doi.org/10.1309/5ADNAR4VN11TTKQ6
Publication status	Published - 1 Jul 2008

Keywords

Adult
Aged
Celiac Disease/pathology
Cell Proliferation
Duodenum/pathology
Female
Humans
Intestinal Mucosa/pathology
Ki-67 Antigen/analysis
Male
Middle Aged
Paneth Cells/pathology
RNA, Messenger/metabolism
alpha-Defensins/analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1309/5ADNAR4VN11TTKQ6

Cite this

Di Sabatino, A., Miceli, E., Dhaliwal, W., Biancheri, P., Salerno, R., Cantoro, L., Vanoli, A., De Vincenzi, M., Blanco, C. D. V., MacDonald, T. T., & Corazza, G. R. (2008). Distribution, proliferation, and function of Paneth cells in uncomplicated and complicated adult celiac disease. American Journal of Clinical Pathology, 130(1), 34-42. https://doi.org/10.1309/5ADNAR4VN11TTKQ6

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abstract = "Paneth cells, granulated epithelial cells located at the base of small bowel crypts, have a crucial role in innate immunity. Because controversies remain concerning Paneth cell numbers and function in celiac disease (CD), we quantified Paneth cells and human alpha-defensin (HD)-5 and HD-6 in 28 patients with uncomplicated CD, 8 patients with complicated CD (3 with ulcerative jejunoileitis, 2 with refractory sprue, and 3 with enteropathy-associated T-cell lymphoma), and 14 control subjects. Paneth cell numbers and proliferation did not differ in uncomplicated untreated and treated CD and control cases. However, the number of Paneth cells was significantly reduced in complicated CD. Mucosal HD-5 and HD-6 were comparable in uncomplicated untreated and treated CD and control cases. Ex vivo gliadin challenge of treated CD biopsy specimens had no effect on mucosal HD-5 and HD-6 transcripts. Paneth cell numbers and alpha-defensins are unchanged in the mucosa in uncomplicated CD. Further studies are needed to clarify the implications of reduction of numbers of Paneth cells in complicated CD.",

keywords = "Adult, Aged, Celiac Disease/pathology, Cell Proliferation, Duodenum/pathology, Female, Humans, Intestinal Mucosa/pathology, Ki-67 Antigen/analysis, Male, Middle Aged, Paneth Cells/pathology, RNA, Messenger/metabolism, alpha-Defensins/analysis",

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Di Sabatino, A, Miceli, E, Dhaliwal, W, Biancheri, P, Salerno, R, Cantoro, L, Vanoli, A, De Vincenzi, M, Blanco, CDV, MacDonald, TT & Corazza, GR 2008, 'Distribution, proliferation, and function of Paneth cells in uncomplicated and complicated adult celiac disease', American Journal of Clinical Pathology, vol. 130, no. 1, pp. 34-42. https://doi.org/10.1309/5ADNAR4VN11TTKQ6

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T1 - Distribution, proliferation, and function of Paneth cells in uncomplicated and complicated adult celiac disease

AU - Di Sabatino, Antonio

AU - Miceli, Emanuela

AU - Dhaliwal, Winnie

AU - Biancheri, Paolo

AU - Salerno, Raffaele

AU - Cantoro, Laura

AU - Vanoli, Alessandro

AU - De Vincenzi, Massimo

AU - Blanco, Camillo Del Vecchio

AU - MacDonald, Thomas T

AU - Corazza, Gino R

PY - 2008/7/1

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N2 - Paneth cells, granulated epithelial cells located at the base of small bowel crypts, have a crucial role in innate immunity. Because controversies remain concerning Paneth cell numbers and function in celiac disease (CD), we quantified Paneth cells and human alpha-defensin (HD)-5 and HD-6 in 28 patients with uncomplicated CD, 8 patients with complicated CD (3 with ulcerative jejunoileitis, 2 with refractory sprue, and 3 with enteropathy-associated T-cell lymphoma), and 14 control subjects. Paneth cell numbers and proliferation did not differ in uncomplicated untreated and treated CD and control cases. However, the number of Paneth cells was significantly reduced in complicated CD. Mucosal HD-5 and HD-6 were comparable in uncomplicated untreated and treated CD and control cases. Ex vivo gliadin challenge of treated CD biopsy specimens had no effect on mucosal HD-5 and HD-6 transcripts. Paneth cell numbers and alpha-defensins are unchanged in the mucosa in uncomplicated CD. Further studies are needed to clarify the implications of reduction of numbers of Paneth cells in complicated CD.

AB - Paneth cells, granulated epithelial cells located at the base of small bowel crypts, have a crucial role in innate immunity. Because controversies remain concerning Paneth cell numbers and function in celiac disease (CD), we quantified Paneth cells and human alpha-defensin (HD)-5 and HD-6 in 28 patients with uncomplicated CD, 8 patients with complicated CD (3 with ulcerative jejunoileitis, 2 with refractory sprue, and 3 with enteropathy-associated T-cell lymphoma), and 14 control subjects. Paneth cell numbers and proliferation did not differ in uncomplicated untreated and treated CD and control cases. However, the number of Paneth cells was significantly reduced in complicated CD. Mucosal HD-5 and HD-6 were comparable in uncomplicated untreated and treated CD and control cases. Ex vivo gliadin challenge of treated CD biopsy specimens had no effect on mucosal HD-5 and HD-6 transcripts. Paneth cell numbers and alpha-defensins are unchanged in the mucosa in uncomplicated CD. Further studies are needed to clarify the implications of reduction of numbers of Paneth cells in complicated CD.

KW - Adult

KW - Aged

KW - Celiac Disease/pathology

KW - Cell Proliferation

KW - Duodenum/pathology

KW - Female

KW - Humans

KW - Intestinal Mucosa/pathology

KW - Ki-67 Antigen/analysis

KW - Male

KW - Middle Aged

KW - Paneth Cells/pathology

KW - RNA, Messenger/metabolism

KW - alpha-Defensins/analysis

U2 - 10.1309/5ADNAR4VN11TTKQ6

DO - 10.1309/5ADNAR4VN11TTKQ6

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C2 - 18550468

VL - 130

SP - 34

EP - 42

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 1943-7722

IS - 1

ER -