Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom

Christian G Giske, Inga Fröding, Chowdhury Mehedi Hasan, Agata Turlej-Rogacka, Mark Toleman, David Livermore, Neil Woodford, Timothy R Walsh

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143 Citations (Scopus)

Abstract

Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).
Original languageEnglish
Pages (from-to)2735-8
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume56
Issue number5
DOIs
Publication statusPublished - May 2012

Keywords

  • Electrophoresis, Gel, Pulsed-Field
  • Gene Transfer, Horizontal
  • Great Britain
  • Humans
  • India
  • Klebsiella pneumoniae
  • Multilocus Sequence Typing
  • Phylogeography
  • Plasmids
  • Polymerase Chain Reaction
  • Replicon
  • Serotyping
  • Sweden
  • beta-Lactam Resistance
  • beta-Lactamases

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