Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom

Christian G Giske; Inga Fröding; Chowdhury Mehedi Hasan; Agata Turlej-Rogacka; Mark Toleman; David Livermore; Neil Woodford; Timothy R Walsh

doi:10.1128/AAC.06142-11

Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom

Christian G Giske, Inga Fröding, Chowdhury Mehedi Hasan, Agata Turlej-Rogacka, Mark Toleman, David Livermore, Neil Woodford, Timothy R Walsh

Research output: Contribution to journal › Article › peer-review

168 Citations (Scopus)

Abstract

Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).

Original language	English
Pages (from-to)	2735-8
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	56
Issue number	5
DOIs	https://doi.org/10.1128/AAC.06142-11
Publication status	Published - May 2012

Keywords

Electrophoresis, Gel, Pulsed-Field
Gene Transfer, Horizontal
Great Britain
Humans
India
Klebsiella pneumoniae
Multilocus Sequence Typing
Phylogeography
Plasmids
Polymerase Chain Reaction
Replicon
Serotyping
Sweden
beta-Lactam Resistance
beta-Lactamases

Access to Document

10.1128/AAC.06142-11

Cite this

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title = "Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom",

abstract = "Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).",

keywords = "Electrophoresis, Gel, Pulsed-Field, Gene Transfer, Horizontal, Great Britain, Humans, India, Klebsiella pneumoniae, Multilocus Sequence Typing, Phylogeography, Plasmids, Polymerase Chain Reaction, Replicon, Serotyping, Sweden, beta-Lactam Resistance, beta-Lactamases",

author = "Giske, {Christian G} and Inga Fr{\"o}ding and Hasan, {Chowdhury Mehedi} and Agata Turlej-Rogacka and Mark Toleman and David Livermore and Neil Woodford and Walsh, {Timothy R}",

year = "2012",

month = may,

doi = "10.1128/AAC.06142-11",

language = "English",

volume = "56",

pages = "2735--8",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

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T1 - Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom

AU - Giske, Christian G

AU - Fröding, Inga

AU - Hasan, Chowdhury Mehedi

AU - Turlej-Rogacka, Agata

AU - Toleman, Mark

AU - Livermore, David

AU - Woodford, Neil

AU - Walsh, Timothy R

PY - 2012/5

Y1 - 2012/5

N2 - Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).

AB - Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).

KW - Electrophoresis, Gel, Pulsed-Field

KW - Gene Transfer, Horizontal

KW - Great Britain

KW - Humans

KW - India

KW - Klebsiella pneumoniae

KW - Multilocus Sequence Typing

KW - Phylogeography

KW - Plasmids

KW - Polymerase Chain Reaction

KW - Replicon

KW - Serotyping

KW - Sweden

KW - beta-Lactam Resistance

KW - beta-Lactamases

U2 - 10.1128/AAC.06142-11

DO - 10.1128/AAC.06142-11

M3 - Article

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SN - 0066-4804

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

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