Projects per year
The binding of calcium and magnesium ions (M2+) by polymers and other macromolecules in aqueous solution is ubiquitous across chemistry and biology. At present, it is difficult to assess the binding affinity of macromolecules for M2+ without recourse to potentiometric titrations and/or isothermal titration calorimetry. Both of these techniques require specialized equipment, and the measurements can be difficult to perform and interpret. Here, we present a new method based on 1H NMR chemical shift imaging (CSI) that enables the binding affinity of polymers to be assessed in a single experiment on standard high-field NMR equipment. In our method, M2+ acetate salt is weighed into a standard 5 mm NMR tube and a solution of polymer layered on top. Dissolution and diffusion of the salt carry the M2+ and acetate ions up through the solution. The concentrations of acetate, [Ac], and free (unbound) M2+, [M2+]f, are measured at different positions along the sample by CSI. Binding of M2+ to the polymer reduces [M2+]f and hinders the upward diffusion of M2+. A discrepancy is thus observed between [Ac] and [M2+]f from which the binding affinity of the polymer can be assessed. For systems which form insoluble complexes with M2+, such as sodium polyacrylate or carboxylate-functionalized nanocellulose (CNC), we can determine the concentration of M2+ at which the polymer will precipitate. We can also predict [M2+]f when a solution of polymer is mixed homogeneously with M2+ salt. We assess the binding properties of sodium polyacrylate, alginate, polystyrene sulfonate, CNC, polyethyleneimine, ethylenediamenetetraacetic acid, and maleate.
|Number of pages||8|
|Early online date||25 Jul 2022|
|Publication status||Published - 9 Aug 2022|
- 1 Finished
An assembly platform for the rational design of cellulose nanocrystalline hydrogels.
15/03/19 → 14/03/20
Data for: Does it bind? a method to determine the affinity of calcium and magnesium ions for polymers using 1H NMR spectroscopy
Wallace, M. (Creator), Holroyd, J. (Creator), Kuraite, A. (Creator) & Hussain, H. (Creator), American Chemical Society, 21 Jul 2022