TY - JOUR
T1 - Dose-dependent effects of enteral nutrition on the faecal microbiota and short chain fatty acids
AU - Jatkowska, Aleksandra
AU - Gkikas, Konstantinos
AU - Nichols, Ben
AU - Short, Bryn
AU - Rizou, Vasiliki Konstantina
AU - Kapranos, Panagiotis
AU - Gunnewiek, Jennifer Klein
AU - Christina, Edelyn
AU - Svolos, Vaios
AU - Quince, Christopher
AU - Gerasimidis, Konstantinos
N1 - Data availability statement: The raw sequencing data used for this project has been deposited in the European Nucleotide Archive (ENA) under accession number: PRJEB72881. Other datasets will be shared upon request.
Funding Information: The studentships of AJ and KGk are funded by Nestle Health Science and the University of Glasgow . KGe received research funding, speakers fee and travel expenses covered by Nestle Health Science , Nutricia-Danone , AbbVie , Janssen , Abbott . VS received consultancy fees from Chronicles Health. The rest of the authors have no conflicts of interest to disclose. The funders had no role in the conception, design, execution, interpretation, writing or submission of this manuscript.
PY - 2024/5
Y1 - 2024/5
N2 - Introduction: Enteral nutrition (EN) involves replacing all or part of a person's habitual diet with a nutritional formula. The impact of varying doses of EN on the gut microbiome remains understudied. Methods: Healthy adults replaced all (100% EN) or part (85% EN, 50% EN and 20% EN) of their energy requirements with EN for 7 days. Faecal samples were collected before and on day 7 of interventions. Faecal pH, short chain fatty acids (SCFAs), branched-chain fatty acids (BCFAs) and 16S rRNA sequencing were performed. Dietary assessment was performed with 7-day food diaries. Results: Sixty-one participants (31 females; median (IQR) age: 24.7 (23.0–27.8) years) were recruited. A dose-dependent impact of EN on faecal microbiota, SCFAs, BCFAs) and pH was observed, with changes detectable at EN intakes of at least 50% of energy requirements. 100% and 85% EN reduced the abundance of fibre-fermenting taxa such as Agathobacter, Faecalibaterium, Succinivibrio and Acidaminococcus. In parallel, potentially harmful organisms like Eubacterium, Actinomyces, and Klebsiella increased. In the 50% EN group, adherence to a diet high in fish, vegetables, potatoes, non-alcoholic beverages, and fat spreads, and low in cereal products, milk, and meat negatively correlated with changes in microbiota structure (r = −0.75, P = 0.025). This signal was not observed when using compositional tools for microbiota analysis. Conclusions: EN detrimentally influences the faecal microbiota and diet-related bacterial metabolites in a dose-dependent manner, particularly at doses of at least 50%. The findings of this study have implications for the dietary management and counselling of patients receiving high volume EN.
AB - Introduction: Enteral nutrition (EN) involves replacing all or part of a person's habitual diet with a nutritional formula. The impact of varying doses of EN on the gut microbiome remains understudied. Methods: Healthy adults replaced all (100% EN) or part (85% EN, 50% EN and 20% EN) of their energy requirements with EN for 7 days. Faecal samples were collected before and on day 7 of interventions. Faecal pH, short chain fatty acids (SCFAs), branched-chain fatty acids (BCFAs) and 16S rRNA sequencing were performed. Dietary assessment was performed with 7-day food diaries. Results: Sixty-one participants (31 females; median (IQR) age: 24.7 (23.0–27.8) years) were recruited. A dose-dependent impact of EN on faecal microbiota, SCFAs, BCFAs) and pH was observed, with changes detectable at EN intakes of at least 50% of energy requirements. 100% and 85% EN reduced the abundance of fibre-fermenting taxa such as Agathobacter, Faecalibaterium, Succinivibrio and Acidaminococcus. In parallel, potentially harmful organisms like Eubacterium, Actinomyces, and Klebsiella increased. In the 50% EN group, adherence to a diet high in fish, vegetables, potatoes, non-alcoholic beverages, and fat spreads, and low in cereal products, milk, and meat negatively correlated with changes in microbiota structure (r = −0.75, P = 0.025). This signal was not observed when using compositional tools for microbiota analysis. Conclusions: EN detrimentally influences the faecal microbiota and diet-related bacterial metabolites in a dose-dependent manner, particularly at doses of at least 50%. The findings of this study have implications for the dietary management and counselling of patients receiving high volume EN.
KW - Crohn's disease
KW - Enteral nutrition
KW - Eosinophilic oesophagitis
KW - Gut microbiome
KW - Nutritional rehabilitation
UR - http://www.scopus.com/inward/record.url?scp=85189945646&partnerID=8YFLogxK
U2 - 10.1016/j.clnu.2024.04.010
DO - 10.1016/j.clnu.2024.04.010
M3 - Article
C2 - 38615449
AN - SCOPUS:85189945646
VL - 43
SP - 1200
EP - 1207
JO - Clinical Nutrition
JF - Clinical Nutrition
SN - 0261-5614
IS - 5
ER -