DySCo: Quantitating associations of membrane proteins using two-color single-molecule tracking

Paul D. Dunne; Ricardo A. Fernandes; James McColl; Won Yoon Ji; John R. James; Simon J. Davis; David Klenerman

doi:10.1016/j.bpj.2009.05.046

DySCo: Quantitating associations of membrane proteins using two-color single-molecule tracking

Paul D. Dunne, Ricardo A. Fernandes, James McColl, Won Yoon Ji, John R. James, Simon J. Davis, David Klenerman

Faculty of Science

Research output: Contribution to journal › Letter › peer-review

61 Citations (Scopus)

Abstract

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.

Original language	English
Pages (from-to)	L5-L7
Number of pages	3
Journal	Biophysical Journal
Volume	97
Issue number	4
Early online date	13 Aug 2009
DOIs	https://doi.org/10.1016/j.bpj.2009.05.046
Publication status	Published - 19 Aug 2009

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10.1016/j.bpj.2009.05.046Licence: CC BY-NC-ND

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abstract = "We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.",

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AU - Dunne, Paul D.

AU - Fernandes, Ricardo A.

AU - McColl, James

AU - Ji, Won Yoon

AU - James, John R.

AU - Davis, Simon J.

AU - Klenerman, David

PY - 2009/8/19

Y1 - 2009/8/19

N2 - We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.

AB - We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.

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DO - 10.1016/j.bpj.2009.05.046

M3 - Letter

VL - 97

SP - L5-L7

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

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ER -

DySCo: Quantitating associations of membrane proteins using two-color single-molecule tracking

Abstract

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