TY - JOUR
T1 - Effects of decavanadate salts with organic and inorganic cations on Escherichia coli, Giardia intestinalis, and vero cells
AU - Missina, Juliana M.
AU - Gavinho, Bruno
AU - Postal, Kahoana
AU - Santana, Francielli S.
AU - Valdameri, Glaucio
AU - De Souza, Emanuel M.
AU - Hughes, David L.
AU - Ramirez, Marcel I.
AU - Soares, Jaísa F.
AU - Nunes, Giovana G.
PY - 2018/10
Y1 - 2018/10
N2 -
Decavanadate salts with nicotinamide (3-pyridinecarboxamide, 3-pca) and isonicotinamide (4-pyridinecarboxamide, 4-pca) in both neutral and protonated forms, (3-Hpca)
4
[H
2
V
10
O
28
]·2H
2
O·2(3-pca) (complex I) and (4-Hpca)
4
[H
2
V
10
O
28
]·2(4-pca) (complex II), have been synthesized and characterized by vibrational spectroscopy (infrared and Raman), thermogravimetric analysis (TGA),
51
V NMR, and single-crystal X-ray diffraction analysis. The effects of sodium decavanadate (henceforth called NaV
10
) and compounds I and II on Escherichia coli, Giardia intestinalis, and Vero (African green monkey epithelial kidney) cells were evaluated. Enhanced growth inhibitory activity against E. coli cultures was observed upon treatment with products I and II when compared to that with NaV
10
(GI
50
values of 2.8, 4.0, and 11 mmol L
-1
, respectively), as well as lower cell viability as measured by the intake of propidium iodide (PI). Exposure of Giardia trophozoites to NaV
10
and II revealed reduction in trophozoite viability (GI
50
values of ca. 10 μmol L
-1
) and affected the parasite adherence to both polystyrene culture tubes and a monolayer of Vero cells, even at low concentrations. A lesser effect on Giardia was shown for I. Furthermore, all three compounds were significantly less toxic to Vero cells than the reference drug, albendazole, employed in the treatment of giardiasis. Toxicity reports of oxidovanadium compounds toward Giardia are unprecedented and open a path to the development of new therapeutic agents.
AB -
Decavanadate salts with nicotinamide (3-pyridinecarboxamide, 3-pca) and isonicotinamide (4-pyridinecarboxamide, 4-pca) in both neutral and protonated forms, (3-Hpca)
4
[H
2
V
10
O
28
]·2H
2
O·2(3-pca) (complex I) and (4-Hpca)
4
[H
2
V
10
O
28
]·2(4-pca) (complex II), have been synthesized and characterized by vibrational spectroscopy (infrared and Raman), thermogravimetric analysis (TGA),
51
V NMR, and single-crystal X-ray diffraction analysis. The effects of sodium decavanadate (henceforth called NaV
10
) and compounds I and II on Escherichia coli, Giardia intestinalis, and Vero (African green monkey epithelial kidney) cells were evaluated. Enhanced growth inhibitory activity against E. coli cultures was observed upon treatment with products I and II when compared to that with NaV
10
(GI
50
values of 2.8, 4.0, and 11 mmol L
-1
, respectively), as well as lower cell viability as measured by the intake of propidium iodide (PI). Exposure of Giardia trophozoites to NaV
10
and II revealed reduction in trophozoite viability (GI
50
values of ca. 10 μmol L
-1
) and affected the parasite adherence to both polystyrene culture tubes and a monolayer of Vero cells, even at low concentrations. A lesser effect on Giardia was shown for I. Furthermore, all three compounds were significantly less toxic to Vero cells than the reference drug, albendazole, employed in the treatment of giardiasis. Toxicity reports of oxidovanadium compounds toward Giardia are unprecedented and open a path to the development of new therapeutic agents.
UR - http://www.scopus.com/inward/record.url?scp=85053492139&partnerID=8YFLogxK
U2 - 10.1021/acs.inorgchem.8b01298
DO - 10.1021/acs.inorgchem.8b01298
M3 - Article
C2 - 30198266
AN - SCOPUS:85053492139
VL - 57
SP - 11930
EP - 11941
JO - Inorganic Chemistry
JF - Inorganic Chemistry
SN - 0020-1669
IS - 19
ER -