Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients

Maria Tomas, Michel Doumith, Marina Warner, Jane F. Turton, Alejandro Beceiro, German Bou, David M. Livermore, Neil Woodford

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)

Abstract

Expression of ampC, oprD, mexA, mexC, mexE, and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA.
Original languageEnglish
Pages (from-to)2219-2224
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume54
Issue number5
DOIs
Publication statusPublished - May 2010

Keywords

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Cystic Fibrosis
  • Drug Resistance, Bacterial
  • Drug Resistance, Multiple
  • Electrophoresis, Gel, Pulsed-Field
  • Humans
  • Membrane Transport Proteins
  • Mutation
  • Porins
  • Pseudomonas Infections
  • Pseudomonas aeruginosa
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation
  • beta-Lactamases

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