Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients

Maria Tomas; Michel Doumith; Marina Warner; Jane F. Turton; Alejandro Beceiro; German Bou; David M. Livermore; Neil Woodford

doi:10.1128/AAC.00816-09

Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients

Maria Tomas, Michel Doumith, Marina Warner, Jane F. Turton, Alejandro Beceiro, German Bou, David M. Livermore, Neil Woodford

Research output: Contribution to journal › Article › peer-review

122 Citations (Scopus)

Abstract

Expression of ampC, oprD, mexA, mexC, mexE, and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA.

Original language	English
Pages (from-to)	2219-2224
Number of pages	6
Journal	Antimicrobial Agents and Chemotherapy
Volume	54
Issue number	5
DOIs	https://doi.org/10.1128/AAC.00816-09
Publication status	Published - May 2010

Keywords

Bacterial Outer Membrane Proteins
Bacterial Proteins
Cystic Fibrosis
Drug Resistance, Bacterial
Drug Resistance, Multiple
Electrophoresis, Gel, Pulsed-Field
Humans
Membrane Transport Proteins
Mutation
Porins
Pseudomonas Infections
Pseudomonas aeruginosa
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
beta-Lactamases

Access to Document

10.1128/AAC.00816-09

Cite this

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title = "Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients",

abstract = "Expression of ampC, oprD, mexA, mexC, mexE, and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA.",

keywords = "Bacterial Outer Membrane Proteins, Bacterial Proteins, Cystic Fibrosis, Drug Resistance, Bacterial, Drug Resistance, Multiple, Electrophoresis, Gel, Pulsed-Field, Humans, Membrane Transport Proteins, Mutation, Porins, Pseudomonas Infections, Pseudomonas aeruginosa, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation, beta-Lactamases",

author = "Maria Tomas and Michel Doumith and Marina Warner and Turton, {Jane F.} and Alejandro Beceiro and German Bou and Livermore, {David M.} and Neil Woodford",

year = "2010",

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language = "English",

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T1 - Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients

AU - Tomas, Maria

AU - Doumith, Michel

AU - Warner, Marina

AU - Turton, Jane F.

AU - Beceiro, Alejandro

AU - Bou, German

AU - Livermore, David M.

AU - Woodford, Neil

PY - 2010/5

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AB - Expression of ampC, oprD, mexA, mexC, mexE, and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA.

KW - Bacterial Outer Membrane Proteins

KW - Bacterial Proteins

KW - Cystic Fibrosis

KW - Drug Resistance, Bacterial

KW - Drug Resistance, Multiple

KW - Electrophoresis, Gel, Pulsed-Field

KW - Humans

KW - Membrane Transport Proteins

KW - Mutation

KW - Porins

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Up-Regulation

KW - beta-Lactamases

U2 - 10.1128/AAC.00816-09

DO - 10.1128/AAC.00816-09

M3 - Article

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VL - 54

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EP - 2224

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

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