Electrode assemblies composed of redox cascades from microbial respiratory electron transfer chains

Andrew J Gates, Sophie J Marritt, Justin M Bradley, Liang Shi, Duncan G G McMillan, Lars J C Jeuken, David J Richardson, Julea N Butt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Respiratory and photosynthetic electron transfer chains are dependent on vectorial electron transfer through a series of redox proteins. Examples include electron transfer from NapC to NapAB nitrate reductase in Paracoccus denitrificans and from CymA to Fcc3 (flavocytochrome c3) fumarate reductase in Shewanella oneidensis MR-1. In the present article, we demonstrate that graphite electrodes can serve as surfaces for the stepwise adsorption of NapC and NapAB, and the stepwise adsorption of CymA and Fcc3. Aspects of the catalytic properties of these assemblies are different from those of NapAB and Fcc3 adsorbed in isolation. We propose that this is due to the formation of NapC-NapAB and of CymA-Fcc3 complexes that are capable of supporting vectorial electron transfer.
Original languageEnglish
Pages (from-to)1249-1253
Number of pages5
JournalBiochemical Society Transactions
Volume41
Issue number5
DOIs
Publication statusPublished - 1 Oct 2013

Keywords

  • CymA
  • fumarate reductase
  • NapC
  • nitrate reductase
  • protein film voltammetry
  • quinone

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