TY - JOUR
T1 - Emergence of resistance to fluoroquinolones and third-generation cephalosporins in Salmonella Typhi in Lahore, Pakistan
AU - Rasheed, Farhan
AU - Saeed, Muhammad
AU - Alikhan, Nabil-Fareed
AU - Baker, David
AU - Khurshid, Mohsin
AU - Ainsworth, Emma V.
AU - Turner, A. Keith
AU - Imran, Ambereen Anwar
AU - Rasool, Muhammad Hidayat
AU - Saqalein, Muhammad
AU - Nisar, Muhammad Atif
AU - Fayyaz Ur Rehman, Muhammad
AU - Wain, John
AU - Yasir, Muhammad
AU - Langridge, Gemma C.
AU - Ikram, Aamer
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Extensively drug-resistant (XDR) Salmonella Typhi has been reported in Sindh province of Pakistan since 2016. The potential for further spread is of serious concern as remaining treatment options are severely limited. We report the phenotypic and genotypic characterization of 27 XDR S. Typhi isolated from patients attending Jinnah Hospital, Lahore, Pakistan. Isolates were identified by biochemical profiling; antimicrobial susceptibility was determined by a modified Kirby-Bauer method. These findings were confirmed using Illumina whole genome nucleotide sequence data. All sequences were compared to the outbreak strain from Southern Pakistan and typed using the S. Typhi genotyping scheme. All isolates were confirmed by a sequence analysis to harbor an IncY plasmid and the CTX-M-15 ceftriaxone resistance determinant. All isolates were of the same genotypic background as the outbreak strain from Sindh province. We report the first emergence of XDR S. Typhi in Punjab province of Pakistan confirmed by whole genome sequencing.
AB - Extensively drug-resistant (XDR) Salmonella Typhi has been reported in Sindh province of Pakistan since 2016. The potential for further spread is of serious concern as remaining treatment options are severely limited. We report the phenotypic and genotypic characterization of 27 XDR S. Typhi isolated from patients attending Jinnah Hospital, Lahore, Pakistan. Isolates were identified by biochemical profiling; antimicrobial susceptibility was determined by a modified Kirby-Bauer method. These findings were confirmed using Illumina whole genome nucleotide sequence data. All sequences were compared to the outbreak strain from Southern Pakistan and typed using the S. Typhi genotyping scheme. All isolates were confirmed by a sequence analysis to harbor an IncY plasmid and the CTX-M-15 ceftriaxone resistance determinant. All isolates were of the same genotypic background as the outbreak strain from Sindh province. We report the first emergence of XDR S. Typhi in Punjab province of Pakistan confirmed by whole genome sequencing.
KW - Cephalosporin resistance
KW - Lahore
KW - Salmonella Typhi
KW - Typhoid fever
KW - XDR
UR - http://www.scopus.com/inward/record.url?scp=85093887595&partnerID=8YFLogxK
U2 - 10.3390/microorganisms8091336
DO - 10.3390/microorganisms8091336
M3 - Article
C2 - 32883020
VL - 8
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 9
M1 - 1336
ER -
