Engineering purple bacterial carotenoid biosynthesis to study the roles of carotenoids in light-harvesting complexes

George A. Sutherland, Pu Qian, C. Neil Hunter, David J. K. Swainsbury, Andrew Hitchcock

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Carotenoids are important photosynthetic pigments that play key roles in light harvesting and energy transfer, photoprotection, and in the folding, assembly, and stabilization of light-harvesting pigment–protein complexes. The genetically tractable purple phototrophic bacteria have been useful for investigating the biosynthesis and function of photosynthetic pigments and cofactors, including carotenoids. Here, we give an overview of the roles of carotenoids in photosynthesis and of their biosynthesis, focusing on the extensively studied purple bacterium Rhodobacter sphaeroides as a model organism. We provide detailed procedures for manipulating carotenoid biosynthesis, and for the preparation and analysis of the light-harvesting and photosynthetic reaction center complexes that bind them. Using appropriate examples from the literature, we discuss how such approaches have enhanced our understanding of the biosynthesis of carotenoids and the photosynthesis-related functions of these fascinating molecules.
Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherElsevier
Number of pages48
DOIs
Publication statusE-pub ahead of print - 20 May 2022

Keywords

  • Carotenoids
  • Chlorophyll
  • Cryogenic electron microscopy (cryo-EM)
  • Light-harvesting complex
  • Neurosporene
  • Photoprotection
  • Photosynthesis
  • Purple bacteria
  • Reaction center
  • Rhodobacter sphaeroides
  • Spheroidene
  • Spheroidenone

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