Enhanced imaging of DNA via active quality factor control

A. D. L. Humphris; A. N. Round; M. J. Miles

doi:10.1016/S0039-6028(01)01312-7

Enhanced imaging of DNA via active quality factor control

A. D. L. Humphris, A. N. Round, M. J. Miles

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Adsorption processes at single molecule level are of fundamental importance for the understanding and development of biomaterials. Atomic force microscopy (AFM) has played a critical role in this field due to its high resolution and ability to image in a liquid environment. We present a method that improves the dynamic force sensitivity and the resolution of a conventional AFM. This is achieved via a positive feedback loop that enhances the effective quality factor of the cantilever in a liquid environment to values in excess of 300, compared to a nominal value of similar to1. This active quality factor enhancement has been used to image DNA and an increase in the height of the molecule observed. (C) 2001 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	468-472
Number of pages	5
Journal	Surface Science
Volume	491
Issue number	3
DOIs	https://doi.org/10.1016/S0039-6028(01)01312-7
Publication status	Published - 2001

Keywords

PLASMID DNA
morphology
LIQUIDS
ATOMIC-FORCE MICROSCOPY
topography
STRANDED-DNA
roughness
atomic force microscopy
biological molecules - nucleic acids
and
surface structure

Access to Document

10.1016/S0039-6028(01)01312-7

Cite this

@article{d17888009dbe4929abbf70fac06baf46,

title = "Enhanced imaging of DNA via active quality factor control",

abstract = "Adsorption processes at single molecule level are of fundamental importance for the understanding and development of biomaterials. Atomic force microscopy (AFM) has played a critical role in this field due to its high resolution and ability to image in a liquid environment. We present a method that improves the dynamic force sensitivity and the resolution of a conventional AFM. This is achieved via a positive feedback loop that enhances the effective quality factor of the cantilever in a liquid environment to values in excess of 300, compared to a nominal value of similar to1. This active quality factor enhancement has been used to image DNA and an increase in the height of the molecule observed. (C) 2001 Elsevier Science B.V. All rights reserved.",

keywords = "PLASMID DNA, morphology, LIQUIDS, ATOMIC-FORCE MICROSCOPY, topography, STRANDED-DNA, roughness, atomic force microscopy, biological molecules - nucleic acids, and, surface structure",

author = "Humphris, {A. D. L.} and Round, {A. N.} and Miles, {M. J.}",

year = "2001",

doi = "10.1016/S0039-6028(01)01312-7",

language = "English",

volume = "491",

pages = "468--472",

journal = "Surface Science",

issn = "0039-6028",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - Enhanced imaging of DNA via active quality factor control

AU - Humphris, A. D. L.

AU - Round, A. N.

AU - Miles, M. J.

PY - 2001

Y1 - 2001

N2 - Adsorption processes at single molecule level are of fundamental importance for the understanding and development of biomaterials. Atomic force microscopy (AFM) has played a critical role in this field due to its high resolution and ability to image in a liquid environment. We present a method that improves the dynamic force sensitivity and the resolution of a conventional AFM. This is achieved via a positive feedback loop that enhances the effective quality factor of the cantilever in a liquid environment to values in excess of 300, compared to a nominal value of similar to1. This active quality factor enhancement has been used to image DNA and an increase in the height of the molecule observed. (C) 2001 Elsevier Science B.V. All rights reserved.

AB - Adsorption processes at single molecule level are of fundamental importance for the understanding and development of biomaterials. Atomic force microscopy (AFM) has played a critical role in this field due to its high resolution and ability to image in a liquid environment. We present a method that improves the dynamic force sensitivity and the resolution of a conventional AFM. This is achieved via a positive feedback loop that enhances the effective quality factor of the cantilever in a liquid environment to values in excess of 300, compared to a nominal value of similar to1. This active quality factor enhancement has been used to image DNA and an increase in the height of the molecule observed. (C) 2001 Elsevier Science B.V. All rights reserved.

KW - PLASMID DNA

KW - morphology

KW - LIQUIDS

KW - ATOMIC-FORCE MICROSCOPY

KW - topography

KW - STRANDED-DNA

KW - roughness

KW - atomic force microscopy

KW - biological molecules - nucleic acids

KW - and

KW - surface structure

U2 - 10.1016/S0039-6028(01)01312-7

DO - 10.1016/S0039-6028(01)01312-7

M3 - Article

VL - 491

SP - 468

EP - 472

JO - Surface Science

JF - Surface Science

SN - 0039-6028

IS - 3

ER -