Enzyme-catalysed nitrate reduction—themes and variations as revealed by protein film voltammetry

Julea N. Butt, Lee J. Anderson, Luis M. Rubio, David J. Richardson, Enrique Flores, Antonia Herrero

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Protein film voltammetry has been used to define the catalytic performance of two nitrate reductases: the respiratory nitrate reductase, NarGH, from Paracoccus pantotrophus and the assimilatory nitrate reductase, NarB, from Synechococcus sp. PCC 7942. NarGH and NarB present distinct ‘fingerprints’ of catalytic activity when viewed in this way. Potentials that provide insufficient driving force for significant rates of nitrate reduction by NarB result in appreciable rates of nitrate reduction by NarGH. However, both enzymes display complex modulations in their rate of substrate reduction when viewed across the electrochemical potential domain.
Original languageEnglish
Pages (from-to)17-18
Number of pages2
JournalBioelectrochemistry
Volume56
Issue number1-2
DOIs
Publication statusPublished - 15 May 2002

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