Ertapenem resistance among Klebsiella and Enterobacter submitted in the UK to a reference laboratory

Neil Woodford; John W. T. Dallow; Robert L. R. Hill; Marie-France I. Palepou; Rachel Pike; M. Elaina Ward; Marina Warner; David M. Livermore

doi:10.1016/j.ijantimicag.2006.11.020

Ertapenem resistance among Klebsiella and Enterobacter submitted in the UK to a reference laboratory

Neil Woodford, John W. T. Dallow, Robert L. R. Hill, Marie-France I. Palepou, Rachel Pike, M. Elaina Ward, Marina Warner, David M. Livermore

Research output: Contribution to journal › Article › peer-review

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Abstract

The emergence of carbapenem resistance in Enterobacteriaceae represents a major public health concern. We investigated ertapenem-resistant clinical isolates of Klebsiella spp. and Enterobacter spp. referred to the UK's national reference laboratory for antibiotic resistance. Minimum inhibitory concentrations (MICs) were determined and interpreted according to British Society for Antimicrobial Chemotherapy guidelines. Genes for carbapenemases and CTX-M extended-spectrum ß-lactamases (ESBLs) were sought by polymerase chain reaction, and imipenem hydrolysis was determined by spectrophotometry with crude extracts. From June 2004 to April 2006, 95 Klebsiella spp. and 76 Enterobacter spp. isolates resistant to ertapenem (MICs >2 mg/L) were received, 40% of which (38 Klebsiella spp. and 30 Enterobacter spp.) were highly resistant to ertapenem (MICs >16 mg/L). Imipenem and meropenem were active (geometric mean MICs

Original language	English
Pages (from-to)	456-459
Number of pages	4
Journal	International Journal of Antimicrobial Agents
Volume	29
Issue number	4
DOIs	https://doi.org/10.1016/j.ijantimicag.2006.11.020
Publication status	Published - Apr 2007

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10.1016/j.ijantimicag.2006.11.020

Cite this

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title = "Ertapenem resistance among Klebsiella and Enterobacter submitted in the UK to a reference laboratory",

abstract = "The emergence of carbapenem resistance in Enterobacteriaceae represents a major public health concern. We investigated ertapenem-resistant clinical isolates of Klebsiella spp. and Enterobacter spp. referred to the UK's national reference laboratory for antibiotic resistance. Minimum inhibitory concentrations (MICs) were determined and interpreted according to British Society for Antimicrobial Chemotherapy guidelines. Genes for carbapenemases and CTX-M extended-spectrum {\ss}-lactamases (ESBLs) were sought by polymerase chain reaction, and imipenem hydrolysis was determined by spectrophotometry with crude extracts. From June 2004 to April 2006, 95 Klebsiella spp. and 76 Enterobacter spp. isolates resistant to ertapenem (MICs >2 mg/L) were received, 40% of which (38 Klebsiella spp. and 30 Enterobacter spp.) were highly resistant to ertapenem (MICs >16 mg/L). Imipenem and meropenem were active (geometric mean MICs ",

author = "Neil Woodford and Dallow, {John W. T.} and Hill, {Robert L. R.} and Palepou, {Marie-France I.} and Rachel Pike and Ward, {M. Elaina} and Marina Warner and Livermore, {David M.}",

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AU - Woodford, Neil

AU - Dallow, John W. T.

AU - Hill, Robert L. R.

AU - Palepou, Marie-France I.

AU - Pike, Rachel

AU - Ward, M. Elaina

AU - Warner, Marina

AU - Livermore, David M.

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AB - The emergence of carbapenem resistance in Enterobacteriaceae represents a major public health concern. We investigated ertapenem-resistant clinical isolates of Klebsiella spp. and Enterobacter spp. referred to the UK's national reference laboratory for antibiotic resistance. Minimum inhibitory concentrations (MICs) were determined and interpreted according to British Society for Antimicrobial Chemotherapy guidelines. Genes for carbapenemases and CTX-M extended-spectrum ß-lactamases (ESBLs) were sought by polymerase chain reaction, and imipenem hydrolysis was determined by spectrophotometry with crude extracts. From June 2004 to April 2006, 95 Klebsiella spp. and 76 Enterobacter spp. isolates resistant to ertapenem (MICs >2 mg/L) were received, 40% of which (38 Klebsiella spp. and 30 Enterobacter spp.) were highly resistant to ertapenem (MICs >16 mg/L). Imipenem and meropenem were active (geometric mean MICs

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