Escherichia coli cytochrome c nitrite reductase NrfA

Thomas A. Clarke; Paul C. Mills; Susie R. Poock; Julea N. Butt; Myles R. Cheesman; Jeffrey A. Cole; Jay C. D. Hinton; Andrew M. Hemmings; Gemma Kemp; Christopher A. G. Söderberg; Stephen Spiro; Jessica van Wonderen; David J. Richardson

doi:10.1016/S0076-6879(07)37004-3

Escherichia coli cytochrome c nitrite reductase NrfA

Thomas A. Clarke, Paul C. Mills, Susie R. Poock, Julea N. Butt, Myles R. Cheesman, Jeffrey A. Cole, Jay C. D. Hinton, Andrew M. Hemmings, Gemma Kemp, Christopher A. G. Söderberg, Stephen Spiro, Jessica van Wonderen, David J. Richardson

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Abstract

The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

Original language	English
Pages (from-to)	63-77
Number of pages	15
Journal	Methods in Enzymology
Volume	437
DOIs	https://doi.org/10.1016/S0076-6879(07)37004-3
Publication status	Published - 2008

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10.1016/S0076-6879(07)37004-3

Cite this

@article{74c17268339e45b4aec4244faa49619a,

title = "Escherichia coli cytochrome c nitrite reductase NrfA",

abstract = "The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.",

author = "Clarke, {Thomas A.} and Mills, {Paul C.} and Poock, {Susie R.} and Butt, {Julea N.} and Cheesman, {Myles R.} and Cole, {Jeffrey A.} and Hinton, {Jay C. D.} and Hemmings, {Andrew M.} and Gemma Kemp and S{\"o}derberg, {Christopher A. G.} and Stephen Spiro and {van Wonderen}, Jessica and Richardson, {David J.}",

year = "2008",

doi = "10.1016/S0076-6879(07)37004-3",

language = "English",

volume = "437",

pages = "63--77",

journal = "Methods in Enzymology",

issn = "1557-7988",

}

TY - JOUR

T1 - Escherichia coli cytochrome c nitrite reductase NrfA

AU - Clarke, Thomas A.

AU - Mills, Paul C.

AU - Poock, Susie R.

AU - Butt, Julea N.

AU - Cheesman, Myles R.

AU - Cole, Jeffrey A.

AU - Hinton, Jay C. D.

AU - Hemmings, Andrew M.

AU - Kemp, Gemma

AU - Söderberg, Christopher A. G.

AU - Spiro, Stephen

AU - van Wonderen, Jessica

AU - Richardson, David J.

PY - 2008

Y1 - 2008

N2 - The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

AB - The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

U2 - 10.1016/S0076-6879(07)37004-3

DO - 10.1016/S0076-6879(07)37004-3

M3 - Article

SN - 1557-7988

VL - 437

SP - 63

EP - 77

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -