TY - JOUR
T1 - Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
AU - Ribeiro, A. P. C.
AU - Anbu, S.
AU - Alegria, E. C. B. A.
AU - Fernandes, A. R.
AU - Baptista, P. V.
AU - Mendes, R.
AU - Matias, A. S.
AU - Mendes, M.
AU - Guedes da Silva, M. F. C.
AU - Pombeiro, A. J. L.
PY - 2018/5
Y1 - 2018/5
N2 - Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV–vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.
AB - Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV–vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-85042378625&partnerID=MN8TOARS
U2 - 10.1016/j.biopha.2018.02.069
DO - 10.1016/j.biopha.2018.02.069
M3 - Article
SN - 1950-6007
VL - 101
SP - 137
EP - 144
JO - Biomedicine and Pharmacotherapy
JF - Biomedicine and Pharmacotherapy
ER -