Evidence against oxidative stress as mechanism of endothelial dysfunction in methionine loading model

Angus K. Nightingale; Philip P. James; Jayne Morris-Thurgood; Fraser Harrold; Richard Tong; Simon K. Jackson; John R. Cockcroft; Michael P. Frenneaux

doi:10.1152/ajpheart.2001.280.3.H1334

Evidence against oxidative stress as mechanism of endothelial dysfunction in methionine loading model

Angus K. Nightingale, Philip P. James, Jayne Morris-Thurgood, Fraser Harrold, Richard Tong, Simon K. Jackson, John R. Cockcroft, Michael P. Frenneaux

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Endothelial dysfunction reflects reduced nitric oxide (NO) bioavailability due to either reduced production, inactivation of NO, or reduced smooth muscle responsiveness. Oral methionine loading causes acute endothelial dysfunction in healthy subjects and provides a model in which to study mechanisms. Endothelial function was assessed using flow-mediated dilatation (FMD) of the brachial artery in humans. Three markers of oxidative stress were measured ex vivo in venous blood. NO responsiveness was assessed in vascular smooth muscle and platelets. Oral methionine loading induced endothelial dysfunction (FMD decreased from 2.8 +/- 0.8 to 0.3 +/- 0.3% with methionine and from 2.8 +/- 0.8 to 1.3 +/- 0.3% with placebo; P

Original language	English
Pages (from-to)	H1334-H1339
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Volume	280
Issue number	3
DOIs	https://doi.org/10.1152/ajpheart.2001.280.3.H1334
Publication status	Published - Mar 2001

Keywords

Adolescent
Adult
Aged
Brachial Artery
Cross-Over Studies
Endothelium, Vascular
Homocysteine
Humans
Lipid Peroxidation
Male
Methionine
Middle Aged
Nitrates
Oxidative Stress
Platelet Aggregation
Prospective Studies
Signal Transduction
Thiobarbituric Acid Reactive Substances

Access to Document

10.1152/ajpheart.2001.280.3.H1334

Cite this

Nightingale, A. K., James, P. P., Morris-Thurgood, J., Harrold, F., Tong, R., Jackson, S. K., Cockcroft, J. R., & Frenneaux, M. P. (2001). Evidence against oxidative stress as mechanism of endothelial dysfunction in methionine loading model. American Journal of Physiology - Heart and Circulatory Physiology, 280(3), H1334-H1339. https://doi.org/10.1152/ajpheart.2001.280.3.H1334

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title = "Evidence against oxidative stress as mechanism of endothelial dysfunction in methionine loading model",

abstract = "Endothelial dysfunction reflects reduced nitric oxide (NO) bioavailability due to either reduced production, inactivation of NO, or reduced smooth muscle responsiveness. Oral methionine loading causes acute endothelial dysfunction in healthy subjects and provides a model in which to study mechanisms. Endothelial function was assessed using flow-mediated dilatation (FMD) of the brachial artery in humans. Three markers of oxidative stress were measured ex vivo in venous blood. NO responsiveness was assessed in vascular smooth muscle and platelets. Oral methionine loading induced endothelial dysfunction (FMD decreased from 2.8 +/- 0.8 to 0.3 +/- 0.3% with methionine and from 2.8 +/- 0.8 to 1.3 +/- 0.3% with placebo; P ",

keywords = "Adolescent, Adult, Aged, Brachial Artery, Cross-Over Studies, Endothelium, Vascular, Homocysteine, Humans, Lipid Peroxidation, Male, Methionine, Middle Aged, Nitrates, Oxidative Stress, Platelet Aggregation, Prospective Studies, Signal Transduction, Thiobarbituric Acid Reactive Substances",

author = "Nightingale, {Angus K.} and James, {Philip P.} and Jayne Morris-Thurgood and Fraser Harrold and Richard Tong and Jackson, {Simon K.} and Cockcroft, {John R.} and Frenneaux, {Michael P.}",

year = "2001",

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doi = "10.1152/ajpheart.2001.280.3.H1334",

language = "English",

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Nightingale, AK, James, PP, Morris-Thurgood, J, Harrold, F, Tong, R, Jackson, SK, Cockcroft, JR & Frenneaux, MP 2001, 'Evidence against oxidative stress as mechanism of endothelial dysfunction in methionine loading model', American Journal of Physiology - Heart and Circulatory Physiology, vol. 280, no. 3, pp. H1334-H1339. https://doi.org/10.1152/ajpheart.2001.280.3.H1334

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AU - Nightingale, Angus K.

AU - James, Philip P.

AU - Morris-Thurgood, Jayne

AU - Harrold, Fraser

AU - Tong, Richard

AU - Jackson, Simon K.

AU - Cockcroft, John R.

AU - Frenneaux, Michael P.

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KW - Lipid Peroxidation

KW - Male

KW - Methionine

KW - Middle Aged

KW - Nitrates

KW - Oxidative Stress

KW - Platelet Aggregation

KW - Prospective Studies

KW - Signal Transduction

KW - Thiobarbituric Acid Reactive Substances

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