Evidence for GC preference by monocot Dicer-like proteins

Thien Ho, Rachel L. Rusholme Pilcher, Mary-Lou Edwards, Ian Cooper, Tamas Dalmay, Hui Wang

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23 Citations (Scopus)


Dicot Dicer-like (DCL) enzymes operate preferably on GC rich regions when producing small interfering (si)RNA and micro (mi)RNA. This GC bias, however, is not generic in monocot miRNA productions. From wild Dactylis glomerata naturally infected by Cocksfoot streak potyvirus (CSV), CSV-siRNAs had a greater GC% than the virus genome, indicating that GC rich regions were also preferred by the grass DCLs. This supports the notion that GC preference is an ancient feature for plant DCLs, and suggests that monocot miRNA genes might have evolved to a high GC% resulting in GC bias being not detectable during mature miRNA production.
Original languageEnglish
Pages (from-to)433-437
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Apr 2008

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