Evidence of metal binding activities of pentadecapeptide from Panax ginseng

Hideyuki Kajiwara; Andrew M. Hemmings; Hisashi Hirano

doi:10.1016/S0378-4347(96)00240-X

Evidence of metal binding activities of pentadecapeptide from Panax ginseng

Hideyuki Kajiwara, Andrew M. Hemmings, Hisashi Hirano

Research output: Contribution to journal › Article › peer-review

Abstract

A tetradecapeptide from ginseng (Panax ginseng) root showing anti-lipolytic activity in an isolated rat fat cell assay was chemically synthesized for analysis of metal binding activities in vitro. Binding activities against several metal ions were analysed by measuring mobility shifts during capillary zone electrophoresis experiments. The ginseng polypeptide (GPP) showed the greatest increase in effective molecular electrophoretic mobility in the presence of Mg²⁺. Mobility was also affected in the presence of La³⁺, Mn²⁺, Ca²⁺ and Zn²⁺ ions. Analysis with the dye Stains-all revealed GPP to possess a cation binding site similar to those in Ca²⁺-binding proteins. GPP thus appears to be a metal binding peptide. The results of this analysis suggested that GPP may perform its anti-lipolytic activities through an ability to modulate the level of free cellular Mg²⁺ and Mn²⁺ ions.

Original language	English
Pages (from-to)	443-448
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Applications
Volume	687
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(96)00240-X
Publication status	Published - 13 Dec 1996

Keywords

Metal binding activity
Metal cations
Panax ginsens
Pentadecapeptide

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10.1016/S0378-4347(96)00240-X

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title = "Evidence of metal binding activities of pentadecapeptide from Panax ginseng",

abstract = "A tetradecapeptide from ginseng (Panax ginseng) root showing anti-lipolytic activity in an isolated rat fat cell assay was chemically synthesized for analysis of metal binding activities in vitro. Binding activities against several metal ions were analysed by measuring mobility shifts during capillary zone electrophoresis experiments. The ginseng polypeptide (GPP) showed the greatest increase in effective molecular electrophoretic mobility in the presence of Mg2+. Mobility was also affected in the presence of La3+, Mn2+, Ca2+ and Zn2+ ions. Analysis with the dye Stains-all revealed GPP to possess a cation binding site similar to those in Ca2+-binding proteins. GPP thus appears to be a metal binding peptide. The results of this analysis suggested that GPP may perform its anti-lipolytic activities through an ability to modulate the level of free cellular Mg2+ and Mn2+ ions.",

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author = "Hideyuki Kajiwara and Hemmings, {Andrew M.} and Hisashi Hirano",

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T1 - Evidence of metal binding activities of pentadecapeptide from Panax ginseng

AU - Kajiwara, Hideyuki

AU - Hemmings, Andrew M.

AU - Hirano, Hisashi

PY - 1996/12/13

Y1 - 1996/12/13

N2 - A tetradecapeptide from ginseng (Panax ginseng) root showing anti-lipolytic activity in an isolated rat fat cell assay was chemically synthesized for analysis of metal binding activities in vitro. Binding activities against several metal ions were analysed by measuring mobility shifts during capillary zone electrophoresis experiments. The ginseng polypeptide (GPP) showed the greatest increase in effective molecular electrophoretic mobility in the presence of Mg2+. Mobility was also affected in the presence of La3+, Mn2+, Ca2+ and Zn2+ ions. Analysis with the dye Stains-all revealed GPP to possess a cation binding site similar to those in Ca2+-binding proteins. GPP thus appears to be a metal binding peptide. The results of this analysis suggested that GPP may perform its anti-lipolytic activities through an ability to modulate the level of free cellular Mg2+ and Mn2+ ions.

AB - A tetradecapeptide from ginseng (Panax ginseng) root showing anti-lipolytic activity in an isolated rat fat cell assay was chemically synthesized for analysis of metal binding activities in vitro. Binding activities against several metal ions were analysed by measuring mobility shifts during capillary zone electrophoresis experiments. The ginseng polypeptide (GPP) showed the greatest increase in effective molecular electrophoretic mobility in the presence of Mg2+. Mobility was also affected in the presence of La3+, Mn2+, Ca2+ and Zn2+ ions. Analysis with the dye Stains-all revealed GPP to possess a cation binding site similar to those in Ca2+-binding proteins. GPP thus appears to be a metal binding peptide. The results of this analysis suggested that GPP may perform its anti-lipolytic activities through an ability to modulate the level of free cellular Mg2+ and Mn2+ ions.

KW - Metal binding activity

KW - Metal cations

KW - Panax ginsens

KW - Pentadecapeptide

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Evidence of metal binding activities of pentadecapeptide from Panax ginseng

Abstract

Keywords

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