Evolution of genome architecture in archaea: Spontaneous generation of a new chromosome in Haloferax volcanii

Darya Ausiannikava, Laura Mitchell, Hannah Marriott, Victoria Smith, Michelle Hawkins, Kira S. Makarova, Eugene V. Koonin, Conrad A. Nieduszynski, Thorsten Allers

Research output: Contribution to journalArticlepeer-review

12 Citations (SciVal)

Abstract

The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evolution of this genome architecture: 1) mutational diversification of a multi-copy chromosome; 2) capture of a new chromosome by horizontal transfer; 3) acquisition of new origins and splitting into two replicationcompetent chromosomes. We report an example of the third scenario: the multi-origin chromosome of the archaeon Haloferax volcanii has split into two elements via homologous recombination. The newly generated elements are bona fide chromosomes, because each bears "chromosomal" replication origins, rRNA loci, and essential genes. The new chromosomes were stable during routine growth but additional genetic manipulation, which involves selective bottlenecks, provoked further rearrangements. To the best of our knowledge, rearrangement of a naturally evolved prokaryotic genome to generate two new chromosomes has not been described previously.

Original languageEnglish
Pages (from-to)1855-1868
Number of pages14
JournalMolecular Biology and Evolution
Volume35
Issue number8
Early online date16 Apr 2018
DOIs
Publication statusPublished - Aug 2018

Keywords

  • Archaea
  • Chromosome
  • Genome architecture
  • Genome stability
  • Haloferax volcanii
  • Homologous recombination
  • Multipartite genome

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