Expression analysis onto microarrays of randomly selected cDNA clones highlights HOXB13 as a marker of human prostate cancer

S. Edwards, C. Campbell, P. Flohr, J. Shipley, I. Giddings, R. Te-Poele, A. Dodson, C. Foster, J. Clark, S. Jhavar, G. Kovacs, C. S. Cooper

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

In a strategy aimed at identifying novel markers of human prostate cancer, we performed expression analysis using microarrays of clones randomly selected from a cDNA library prepared from the LNCaP prostate cancer cell line. Comparisons of expression profiles in primary human prostate cancer adjacent normal prostate tissue, and a selection of other (nonprostate) normal human tissues, led to the identification of a set of clones that were judged as the best candidate markers of normal and/or malignant prostate tissue, DNA sequencing of the selected clones revealed that they included 10 genes that had previously been established as prostate markers: NKX3.1, KLK2, KLK3 (PSA), FOLH1 (PSMA), STEAP2, PSGR, PRAC, RDH11, Prostein and FASN. Following analysis of the expression patterns of all selected and sequenced genes through interrogation of SAGE databases, a further three genes from our clone set, HOXB13, SPON2 and NCAM2, emerged as additional candidate markers of human prostate cancer. Quantitative RT-PCR demonstrated the specificity of expression of HOXB13 in prostate tissue and revealed its ubiquitous expression in a series of 37 primary prostate cancers and 20 normal prostates. These results demonstrate the utility of this expression-microarray approach in hunting for new markers of individual human cancer types.

Original languageEnglish
Pages (from-to)376-381
Number of pages6
JournalBritish Journal of Cancer
Volume92
Issue number2
DOIs
Publication statusPublished - 7 Dec 2004

Keywords

  • Expression
  • HOXB13
  • Microarray
  • Prostate

Cite this