Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

Xiaoxuan Qi; Wenjian Wang; Haohao Dong; Yuying Liang; Changjiang Dong; Hinh Ly

doi:10.1007/978-1-4939-6981-4_12

Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Dong, Hinh Ly

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

Original language	English
Title of host publication	Hemorrhagic Fever Viruses
Subtitle of host publication	Methods and Protocols
Editors	Maria S. Salvato
Publisher	Humana Press
Pages	179-188
Number of pages	10
ISBN (Electronic)	978-1-4939-6981-4
ISBN (Print)	978-1-4939-6980-7
DOIs	https://doi.org/10.1007/978-1-4939-6981-4_12
Publication status	Published - 7 Oct 2017

Publication series

Name	Hemorrhagic Fever Viruses
Volume	1604
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-6981-4_12

http://link.springer.com/10.1007/978-1-4939-6981-4_12

Cite this

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title = "Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus",

abstract = "We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 {\AA}. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 {\AA}, c = 56.40 {\AA}, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.",

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T1 - Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

AU - Qi, Xiaoxuan

AU - Wang, Wenjian

AU - Dong, Haohao

AU - Liang, Yuying

AU - Dong, Changjiang

AU - Ly, Hinh

PY - 2017/10/7

Y1 - 2017/10/7

N2 - We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

AB - We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

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BT - Hemorrhagic Fever Viruses

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