Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Dong, Hinh Ly

Research output: Chapter in Book/Report/Conference proceedingChapter


We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.
Original languageEnglish
Title of host publicationHemorrhagic Fever Viruses
Subtitle of host publicationMethods and Protocols
EditorsMaria S. Salvato
PublisherHumana Press
Number of pages10
ISBN (Electronic)978-1-4939-6981-4
ISBN (Print)978-1-4939-6980-7
Publication statusPublished - 7 Oct 2017

Publication series

NameHemorrhagic Fever Viruses
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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