Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes.

David A. Young; Svetlana Gavrilov; Caroline J. Pennington; Robert K. Nuttall; Dylan R. Edwards; Richard N. Kitsis; Ian M. Clark

doi:10.1016/j.bbrc.2004.07.178

Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes.

David A. Young, Svetlana Gavrilov, Caroline J. Pennington, Robert K. Nuttall, Dylan R. Edwards, Richard N. Kitsis, Ian M. Clark

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

Original language	English
Pages (from-to)	759-765
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	322
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.07.178
Publication status	Published - 2004

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10.1016/j.bbrc.2004.07.178

Cite this

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title = "Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes.",

abstract = "P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.",

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AU - Young, David A.

AU - Gavrilov, Svetlana

AU - Pennington, Caroline J.

AU - Nuttall, Robert K.

AU - Edwards, Dylan R.

AU - Kitsis, Richard N.

AU - Clark, Ian M.

PY - 2004

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N2 - P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

AB - P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

U2 - 10.1016/j.bbrc.2004.07.178

DO - 10.1016/j.bbrc.2004.07.178

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VL - 322

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JF - Biochemical and Biophysical Research Communications

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