EXPRSS: an Illumina based high-throughput expression-profiling method to reveal transcriptional dynamics

Ghanasyam Rallapalli, Eric M Kemen, Alexandre Robert-Seilaniantz, Cécile Segonzac, Graham J Etherington, Kee Hoon Sohn, Daniel MacLean, Jonathan D G Jones

Research output: Contribution to journalArticle

24 Citations (Scopus)
6 Downloads (Pure)

Abstract

Background: Next Generation Sequencing technologies have facilitated differential gene expression analysis through RNA-seq and Tag-seq methods. RNA-seq has biases associated with transcript lengths, lacks uniform coverage of regions in mRNA and requires 10–20 times more reads than a typical Tag-seq. Most existing Tag-seq methods either have biases or not high throughput due to use of restriction enzymes or enzymatic manipulation of 5’ ends of mRNA or use of RNA ligations. 

Results: We have developed EXpression Profiling through Randomly Sheared cDNA tag Sequencing (EXPRSS) that employs acoustic waves to randomly shear cDNA and generate sequence tags at a relatively defined position (~150-200 bp) from the 3′ end of each mRNA. Implementation of the method was verified through comparative analysis of expression data generated from EXPRSS, NlaIII-DGE and Affymetrix microarray and through qPCR quantification of selected genes. EXPRSS is a strand specific and restriction enzyme independent tag sequencing method that does not require cDNA length-based data transformations. EXPRSS is highly reproducible, is high-throughput and it also reveals alternative polyadenylation and polyadenylated antisense transcripts. It is cost-effective using barcoded multiplexing, avoids the biases of existing SAGE and derivative methods and can reveal polyadenylation position from paired-end sequencing. 

Conclusions: EXPRSS Tag-seq provides sensitive and reliable gene expression data and enables high-throughput expression profiling with relatively simple downstream analysis.

Original languageEnglish
Article number341
JournalBMC Genomics
Volume15
DOIs
Publication statusPublished - 6 May 2014

Keywords

  • Next generation sequencing
  • Tag-seq
  • High throughput expression profiling
  • RNA-seq
  • EXPRSS

Cite this