Extensive DNA inversions in the B. fragilis genome control variable gene expression

Ana M. Cerdeño-Tárraga; Sheila Patrick; Lisa C. Crossman; Garry Blakely; Val Abratt; Nicola Lennard; Ian Poxton; Brian Duerden; Barbara Harris; Mike A. Quail; Andrew Barron; Louise Clark; Craig Corton; Jonathan Doggett; Matthew T.G. Holden; Natasha Larke; Alexandra Line; Angela Lord; Halina Norbertczak; Doug Ormond; Claire Price; Ester Rabbinowitsch; John Woodward; Bart Barrell; Julian Parkhill

doi:10.1126/science.1107008

Extensive DNA inversions in the B. fragilis genome control variable gene expression

Ana M. Cerdeño-Tárraga, Sheila Patrick, Lisa C. Crossman, Garry Blakely, Val Abratt, Nicola Lennard, Ian Poxton, Brian Duerden, Barbara Harris, Mike A. Quail, Andrew Barron, Louise Clark, Craig Corton, Jonathan Doggett, Matthew T.G. Holden, Natasha Larke, Alexandra Line, Angela Lord, Halina Norbertczak, Doug OrmondClaire Price, Ester Rabbinowitsch, John Woodward, Bart Barrell, Julian Parkhill

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

237 Citations (Scopus)

Abstract

The obligately anaerobic bacterium Bacteroides fragilis, an opportunistic pathogen and inhabitant of the normal human colonic microbiota, exhibits considerable within-strain phase and antigenic variation of surface components. The complete genome sequence has revealed an unusual breadth (in number and in effect) of DNA inversion events that potentially control expression of many different components, including surface and secreted components, regulatory molecules, and restriction-modification proteins. Invertible promoters of two different types (12 group 1 and 11 group 2) were identified. One group has inversion crossover (fix) sites similar to the hix sites of Salmonella typhimurium. There are also four independent intergenic shufflons that potentially alter the expression and function of varied genes. The composition of the 10 different polysaccharide biosynthesis gene dusters identified (7 with associated invertible promoters) suggests a mechanism of synthesis similar to the O-antigen capsules of Escherichia coli.

Original language	English
Pages (from-to)	1463-1465
Number of pages	3
Journal	Science
Volume	307
Issue number	5714
DOIs	https://doi.org/10.1126/science.1107008
Publication status	Published - 4 Mar 2005

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Cerdeño-Tárraga, A. M., Patrick, S., Crossman, L. C., Blakely, G., Abratt, V., Lennard, N., Poxton, I., Duerden, B., Harris, B., Quail, M. A., Barron, A., Clark, L., Corton, C., Doggett, J., Holden, M. T. G., Larke, N., Line, A., Lord, A., Norbertczak, H., ... Parkhill, J. (2005). Extensive DNA inversions in the B. fragilis genome control variable gene expression. Science, 307(5714), 1463-1465. https://doi.org/10.1126/science.1107008

Cerdeño-Tárraga, Ana M. ; Patrick, Sheila ; Crossman, Lisa C. ; Blakely, Garry ; Abratt, Val ; Lennard, Nicola ; Poxton, Ian ; Duerden, Brian ; Harris, Barbara ; Quail, Mike A. ; Barron, Andrew ; Clark, Louise ; Corton, Craig ; Doggett, Jonathan ; Holden, Matthew T.G. ; Larke, Natasha ; Line, Alexandra ; Lord, Angela ; Norbertczak, Halina ; Ormond, Doug ; Price, Claire ; Rabbinowitsch, Ester ; Woodward, John ; Barrell, Bart ; Parkhill, Julian. / Extensive DNA inversions in the B. fragilis genome control variable gene expression. In: Science. 2005 ; Vol. 307, No. 5714. pp. 1463-1465.

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title = "Extensive DNA inversions in the B. fragilis genome control variable gene expression",

abstract = "The obligately anaerobic bacterium Bacteroides fragilis, an opportunistic pathogen and inhabitant of the normal human colonic microbiota, exhibits considerable within-strain phase and antigenic variation of surface components. The complete genome sequence has revealed an unusual breadth (in number and in effect) of DNA inversion events that potentially control expression of many different components, including surface and secreted components, regulatory molecules, and restriction-modification proteins. Invertible promoters of two different types (12 group 1 and 11 group 2) were identified. One group has inversion crossover (fix) sites similar to the hix sites of Salmonella typhimurium. There are also four independent intergenic shufflons that potentially alter the expression and function of varied genes. The composition of the 10 different polysaccharide biosynthesis gene dusters identified (7 with associated invertible promoters) suggests a mechanism of synthesis similar to the O-antigen capsules of Escherichia coli.",

author = "Cerde{\~n}o-T{\'a}rraga, {Ana M.} and Sheila Patrick and Crossman, {Lisa C.} and Garry Blakely and Val Abratt and Nicola Lennard and Ian Poxton and Brian Duerden and Barbara Harris and Quail, {Mike A.} and Andrew Barron and Louise Clark and Craig Corton and Jonathan Doggett and Holden, {Matthew T.G.} and Natasha Larke and Alexandra Line and Angela Lord and Halina Norbertczak and Doug Ormond and Claire Price and Ester Rabbinowitsch and John Woodward and Bart Barrell and Julian Parkhill",

year = "2005",

month = mar,

day = "4",

doi = "10.1126/science.1107008",

language = "English",

volume = "307",

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Cerdeño-Tárraga, AM, Patrick, S, Crossman, LC, Blakely, G, Abratt, V, Lennard, N, Poxton, I, Duerden, B, Harris, B, Quail, MA, Barron, A, Clark, L, Corton, C, Doggett, J, Holden, MTG, Larke, N, Line, A, Lord, A, Norbertczak, H, Ormond, D, Price, C, Rabbinowitsch, E, Woodward, J, Barrell, B & Parkhill, J 2005, 'Extensive DNA inversions in the B. fragilis genome control variable gene expression', Science, vol. 307, no. 5714, pp. 1463-1465. https://doi.org/10.1126/science.1107008

Extensive DNA inversions in the B. fragilis genome control variable gene expression. / Cerdeño-Tárraga, Ana M.; Patrick, Sheila; Crossman, Lisa C.; Blakely, Garry; Abratt, Val; Lennard, Nicola; Poxton, Ian; Duerden, Brian; Harris, Barbara; Quail, Mike A.; Barron, Andrew; Clark, Louise; Corton, Craig; Doggett, Jonathan; Holden, Matthew T.G.; Larke, Natasha; Line, Alexandra; Lord, Angela; Norbertczak, Halina; Ormond, Doug; Price, Claire; Rabbinowitsch, Ester; Woodward, John; Barrell, Bart; Parkhill, Julian.

In: Science, Vol. 307, No. 5714, 04.03.2005, p. 1463-1465.

Research output: Contribution to journal › Article › peer-review

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AU - Patrick, Sheila

AU - Crossman, Lisa C.

AU - Blakely, Garry

AU - Abratt, Val

AU - Lennard, Nicola

AU - Poxton, Ian

AU - Duerden, Brian

AU - Harris, Barbara

AU - Quail, Mike A.

AU - Barron, Andrew

AU - Clark, Louise

AU - Corton, Craig

AU - Doggett, Jonathan

AU - Holden, Matthew T.G.

AU - Larke, Natasha

AU - Line, Alexandra

AU - Lord, Angela

AU - Norbertczak, Halina

AU - Ormond, Doug

AU - Price, Claire

AU - Rabbinowitsch, Ester

AU - Woodward, John

AU - Barrell, Bart

AU - Parkhill, Julian

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AB - The obligately anaerobic bacterium Bacteroides fragilis, an opportunistic pathogen and inhabitant of the normal human colonic microbiota, exhibits considerable within-strain phase and antigenic variation of surface components. The complete genome sequence has revealed an unusual breadth (in number and in effect) of DNA inversion events that potentially control expression of many different components, including surface and secreted components, regulatory molecules, and restriction-modification proteins. Invertible promoters of two different types (12 group 1 and 11 group 2) were identified. One group has inversion crossover (fix) sites similar to the hix sites of Salmonella typhimurium. There are also four independent intergenic shufflons that potentially alter the expression and function of varied genes. The composition of the 10 different polysaccharide biosynthesis gene dusters identified (7 with associated invertible promoters) suggests a mechanism of synthesis similar to the O-antigen capsules of Escherichia coli.

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U2 - 10.1126/science.1107008

DO - 10.1126/science.1107008

M3 - Article

C2 - 15746427

AN - SCOPUS:20044387585

VL - 307

SP - 1463

EP - 1465

JO - Science

JF - Science

SN - 0036-8075

IS - 5714

ER -

Extensive DNA inversions in the B. fragilis genome control variable gene expression

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