TY - JOUR
T1 - Extreme resistance to Potato virus Y in potato carrying the Rysto gene is mediated by a TIR-NLR immune receptor
AU - Grech-Baran, Marta
AU - Witek, Kamil
AU - Szajko, Katarzyna
AU - Witek, Agnieszka I
AU - Morgiewicz, Karolina
AU - Wasilewicz-Flis, Iwona
AU - Jakuczun, Henryka
AU - Marczewski, Waldemar
AU - Jones, Jonathan D G
AU - Hennig, Jacek
PY - 2020/3
Y1 - 2020/3
N2 - Potato virus Y (PVY) is a major potato (Solanum tuberosum L.) pathogen that causes severe annual crop losses worth billions of dollars worldwide. PVY is transmitted by aphids, and successful control of virus transmission requires the extensive use of environmentally damaging insecticides to reduce vector populations. Rysto , from the wild relative S. stoloniferum, confers extreme resistance (ER) to PVY and related viruses and is a valuable trait that is widely employed in potato resistance breeding programmes. Rysto was previously mapped to a region of potato chromosome XII, but the specific gene has not been identified to date. In this study, we isolated Rysto using resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences single-molecule real-time sequencing). Rysto was found to encode a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal TIR domain and was sufficient for PVY perception and ER in transgenic potato plants. Rysto -dependent extreme resistance was temperature-independent and requires EDS1 and NRG1 proteins. Rysto may prove valuable for creating PVY-resistant cultivars of potato and other Solanaceae crops.
AB - Potato virus Y (PVY) is a major potato (Solanum tuberosum L.) pathogen that causes severe annual crop losses worth billions of dollars worldwide. PVY is transmitted by aphids, and successful control of virus transmission requires the extensive use of environmentally damaging insecticides to reduce vector populations. Rysto , from the wild relative S. stoloniferum, confers extreme resistance (ER) to PVY and related viruses and is a valuable trait that is widely employed in potato resistance breeding programmes. Rysto was previously mapped to a region of potato chromosome XII, but the specific gene has not been identified to date. In this study, we isolated Rysto using resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences single-molecule real-time sequencing). Rysto was found to encode a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal TIR domain and was sufficient for PVY perception and ER in transgenic potato plants. Rysto -dependent extreme resistance was temperature-independent and requires EDS1 and NRG1 proteins. Rysto may prove valuable for creating PVY-resistant cultivars of potato and other Solanaceae crops.
KW - PVY
KW - RenSeq
KW - Ry
KW - TIR-NLR immune receptor
KW - extreme resistance
KW - potato
UR - http://www.scopus.com/inward/record.url?scp=85071778338&partnerID=8YFLogxK
U2 - 10.1111/pbi.13230
DO - 10.1111/pbi.13230
M3 - Article
C2 - 31397954
SN - 1467-7644
VL - 18
SP - 655
EP - 667
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
IS - 3
ER -