F‐κ B modulators from Valeriana officinalis

Valeriana officinalis (Valerianaceae) has been of great interest for its therapeutic uses for treating mild nervous tension and temporary sleeping problems. In traditional European medicine it has been also reported as an antiinflammatory remedy. This study reports that the EtOAc extract of the underground parts of V. officinalis showed inhibitory activity against NF‐κ B at 100 µg/mL in the IL‐6/Luc assay on HeLa cells and provided protection against excitotoxicity in primary brain cell cultures at micromolar concentrations. Bioassay‐guided fractionation of the EtOAc extract led to the isolation of three known sesquiterpenes: acetylvalerenolic acid (1), valerenal (2) and valerenic acid (3), 1 and 3 were active as inhibitors of NF‐κ B at a concentration of 100 µg/mL. Acetylvalerenolic acid (1) reduced NF‐κ B activity to 4%, whereas valerenic acid (3) reduced NF‐κ B activity to 25%.