Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid beta-oxidation: functional role of peroxisome proliferator activated receptor alpha in human peripheral blood mononuclear cells

Mark Bouwens, Lydia A Afman, Michael Müller

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells (PBMCs) are the only readily available cells in healthy humans. Various studies showed disease-characteristic gene expression patterns in PBMCs. However, little is known of nutritional effects on PBMC gene expression patterns. Fatty acids are nutrients that regulate gene expression by activating the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha). PBMCs express PPARalpha, making these cells interesting to study FA-dependent gene expression.
Original languageEnglish
Pages (from-to)1515-23
Number of pages9
JournalAmerican Journal of Clinical Nutrition
Volume86
Issue number5
Publication statusPublished - Nov 2007

Keywords

  • Adult
  • Cells, Cultured
  • Fasting
  • Fatty Acids
  • Fatty Acids, Nonesterified
  • Gene Expression Profiling
  • Humans
  • Leukocytes, Mononuclear
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Oxidation-Reduction
  • PPAR alpha
  • Polymerase Chain Reaction

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