Fetal gut laser microdissection in combination with RNA preamplification enables epithelial-specific transcriptional profiling

J Hemmerling, J Jansen, M Müller, D Haller

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Laser microdissection (LMD) technology enables highly specific gene expression analyses of biologically relevant questions at cell- or tissue-specific resolution. Nevertheless, specific cell types are often limited in quantity (i.e. fetal tissue), making high quality RNA extraction and subsequent gene expression approaches via common reverse transcriptase-quantitative PCR (RT-q-PCR) challenging. In the case of fetal gut epithelia representing immune modulatory interphases gene expression analysis with common RT-q-PCR is limited to a few genes (
Original languageEnglish
Pages (from-to)189–192
JournalJournal of Immunological Methods
Volume416
Early online date24 Nov 2014
DOIs
Publication statusPublished - Jan 2015

Keywords

  • Fetal intestinal epithelium
  • Laser microdissection
  • Whole transcriptome preamplification

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