Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus

Timothy Goodman, Stuart G. Nayar, Shaun Clare, Marta Mikolajczak, Ritva Rice, Suzanne Mansour, Saverio Bellusci, Mohammad K. Hajihosseini

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9 Citations (Scopus)
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Abstract

New neurons are generated in the postnatal rodent hypothalamus, with a subset of tanycytes in the third ventricular (3V) wall serving as neural stem/progenitor cells. However, the precise stem cell niche organization, the intermediate steps and the endogenous regulators of postnatal hypothalamic neurogenesis remain elusive. Quantitative lineage-tracing in vivo revealed that conditional deletion of fibroblast growth factor 10 (Fgf10) from Fgf10-expressing β-tanycytes at postnatal days (P)4-5 results in the generation of significantly more parenchymal cells by P28, composed mostly of ventromedial and dorsomedial neurons and some glial cells, which persist into adulthood. A closer scrutiny in vivo and ex vivo revealed that the 3V wall is not static and is amenable to cell movements. Furthermore, normally β-tanycytes give rise to parenchymal cells via an intermediate population of α-tanycytes with transient amplifying cell characteristics. Loss of Fgf10 temporarily attenuates the amplification of β-tanycytes but also appears to delay the exit of their α-tanycyte descendants from the germinal 3V wall. Our findings suggest that transience of cells through the α-tanycyte domain is a key feature, and Fgf10 is a negative regulator of postnatal hypothalamic neurogenesis.
Original languageEnglish
Article number180950
JournalDevelopment
Volume147
Issue number13
DOIs
Publication statusPublished - 13 Jul 2020

Keywords

  • Cell movement
  • Fibroblast growth factors
  • Hypothalamus
  • Lineage tracing
  • Mouse
  • Postnatal neurogenesis
  • Tanycytes

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