Flavonoids from Artemisia rupestris and their synergistic antibacterial effects on drug-resistant Staphylococcus aureus

Jiang-Er Lan, Xiao-Jin Li, Xiao-Feng Zhu, Zhong-Lin Sun, Jian-Ming He, Mire Zloh, Simon Gibbons, Qing Mu

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    42 Citations (SciVal)

    Abstract

    This study seeks to discover flavonoids from a traditional Chinese herb, Artemisia rupestris L., with synergistic antibacterial effects against multidrug-resistant Staphylococcus aureus. Five flavonoids, artemetin (1), chrysosplenetin (2), pachypodol (3), penduletin (4) and chrysoeriol (5) were obtained by various column chromatographic methods. Their chemical structures were determined on the basis of comprehensive spectroscopic analysis and comparison with literature data. Three of the compounds (2, 4 and 5) exhibited synergistic activity when combined with norfloxacin against SA1199B, an effluxing fluoroquinolone-resistant strain. The fractional inhibitory concentration indices (FICIs) of 2, 4 and 5 in combination with norfloxacin were 0.375, 0.079 and 0.266 respectively, suggesting synergy. Compound 5 also showed synergistic effects against EMRSA-15 and EMRSA-16 when combined with ciprofloxacin and oxacillin exhibiting FICIs of 0.024 and 0.375 respectively. Real time ethidium bromide (EtBr) efflux assay, qRT-PCR and molecular docking were employed to explore the mechanisms of the synergistic effects.

    Original languageEnglish
    Pages (from-to)1881-1886
    Number of pages6
    JournalNatural Product Research
    Volume35
    Issue number11
    Early online date13 Jul 2019
    DOIs
    Publication statusPublished - 2021

    Keywords

    • Artemisia rupestrisL
    • efflux inhibitor
    • flavonoids
    • molecular docking
    • multidrug-resistant Staphylococcus aureus
    • synergism

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