Fluorescence lifetime imaging of quantum dot labeled DNA microarrays

Gerard Giraud; Holger Schulze; Till T. Bachmann; Colin J. Campbell; Andrew R. Mount; Peter Ghazal; Mizanur R. Khondoker; Alan J. Ross; Stuart W. J. Ember; Ilenia Ciani; Chaker Tlili; Anthony J. Walton; Jonathan G. Terry; Jason Crain

doi:10.3390/ijms10041930

Fluorescence lifetime imaging of quantum dot labeled DNA microarrays

Gerard Giraud, Holger Schulze, Till T. Bachmann, Colin J. Campbell, Andrew R. Mount, Peter Ghazal, Mizanur R. Khondoker, Alan J. Ross, Stuart W. J. Ember, Ilenia Ciani, Chaker Tlili, Anthony J. Walton, Jonathan G. Terry, Jason Crain

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38 Citations (Scopus)

Abstract

Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.

Original language	English
Pages (from-to)	1930-1941
Number of pages	12
Journal	International Journal of Molecular Sciences
Volume	10
Issue number	4
DOIs	https://doi.org/10.3390/ijms10041930
Publication status	Published - 24 Apr 2009

Keywords

Quantum Dots (QD)
Fluorescence Lifetime Imaging Microscopy (FLIM)
DNA Microarray
Lifetime Multiplexing

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10.3390/ijms10041930Licence: CC BY

Cite this

Giraud, G., Schulze, H., Bachmann, T. T., Campbell, C. J., Mount, A. R., Ghazal, P., Khondoker, M. R., Ross, A. J., Ember, S. W. J., Ciani, I., Tlili, C., Walton, A. J., Terry, J. G., & Crain, J. (2009). Fluorescence lifetime imaging of quantum dot labeled DNA microarrays. International Journal of Molecular Sciences, 10(4), 1930-1941. https://doi.org/10.3390/ijms10041930

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abstract = "Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.",

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