Fluorescent macrocyclic probes with pendant functional groups as markers of acidic organelles within live cells

Prashant D. Wadhavane, M. Ángeles Izquierdo, Dennis Lutters, M. Isabel Burguete, MJ Marin, David A. Russell, Francisco Galindo, Santiago V. Luis

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A new family of acidity sensitive fluorescent macrocycles has been synthesized and fully characterized. Their photophysical properties including emission quantum yield and fluorescence lifetime have been determined. The acid–base properties of the new molecules can be tuned by the incorporation of pendant functional groups. The nature of such functional groups (carboxylic acid or ester) influences dramatically the pKa of the probes, two compounds of which exhibit low values. Preliminary intracellular studies using confocal microscopy together with emission spectra of the probes from the cellular environment have shown that the synthesized fluorescent macrocycles mark the acidic organelles of RAW 264.7 macrophage cells.
Original languageEnglish
Pages (from-to)823-831
Number of pages9
JournalOrganic & Biomolecular Chemistry
Volume12
DOIs
Publication statusPublished - 1 Jan 2014

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