Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

Moataz Abd El Ghany; Lars Barquist; Simon Clare; Cordelia Brandt; Matthew Mayho; Enrique Joffré; Åsa Sjöling; A. Keith Turner; John D. Klena; Robert A. Kingsley; Grant A. Hill-Cawthorne; Gordon Dougan; Derek Pickard

doi:10.1099/MGEN.0.000554

Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

Moataz Abd El Ghany, Lars Barquist, Simon Clare, Cordelia Brandt, Matthew Mayho, Enrique Joffré, Åsa Sjöling, A. Keith Turner, John D. Klena, Robert A. Kingsley, Grant A. Hill-Cawthorne, Gordon Dougan, Derek Pickard

School of Biological Sciences

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Abstract

Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.

Original language	English
Article number	000554
Journal	Microbial Genomics
Volume	7
Issue number	6
DOIs	https://doi.org/10.1099/MGEN.0.000554
Publication status	Published - 10 Jun 2021

Keywords

CFA/I
ETEC
H10407
RNAseq
TraDIS
Water survival and colonization

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10.1099/MGEN.0.000554Licence: CC BY-NC

mgen000554Final published version, 2.28 MBLicence: CC BY-NC

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Ghany, M. A. E., Barquist, L., Clare, S., Brandt, C., Mayho, M., Joffré, E., Sjöling, Å., Turner, A. K., Klena, J. D., Kingsley, R. A., Hill-Cawthorne, G. A., Dougan, G., & Pickard, D. (2021). Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization. Microbial Genomics, 7(6), [000554]. https://doi.org/10.1099/MGEN.0.000554

@article{6527f492dc59443e81bc86c6a3f0fee4,

title = "Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization",

abstract = "Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.",

keywords = "CFA/I, ETEC, H10407, RNAseq, TraDIS, Water survival and colonization",

author = "Ghany, {Moataz Abd El} and Lars Barquist and Simon Clare and Cordelia Brandt and Matthew Mayho and Enrique Joffr{\'e} and {\AA}sa Sj{\"o}ling and Turner, {A. Keith} and Klena, {John D.} and Kingsley, {Robert A.} and Hill-Cawthorne, {Grant A.} and Gordon Dougan and Derek Pickard",

note = "Funding Information: This work was supported by The Wellcome Trust (G.D.) and Marie Bashir Institute and Sydney Medical School foundation (M.A. and G.A.H.). The work was also supported by an ESPRC Vaccine-Hub grant and the NIMR Cambridge BRC Antibiotic Resistance theme (G.D.) and the Swedish Research Council ({\AA}.S.). R.A.K. was funded by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent project BBS/E/F/000PR10348. The funders had no role in study design, data collection and interpretation or the decision to submit the work for publication.",

year = "2021",

month = jun,

day = "10",

doi = "10.1099/MGEN.0.000554",

language = "English",

volume = "7",

journal = "Microbial Genomics",

issn = "2057-5858",

publisher = "Microbiology Society",

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Ghany, MAE, Barquist, L, Clare, S, Brandt, C, Mayho, M, Joffré, E, Sjöling, Å, Turner, AK, Klena, JD, Kingsley, RA, Hill-Cawthorne, GA, Dougan, G & Pickard, D 2021, 'Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization', Microbial Genomics, vol. 7, no. 6, 000554. https://doi.org/10.1099/MGEN.0.000554

TY - JOUR

T1 - Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

AU - Ghany, Moataz Abd El

AU - Barquist, Lars

AU - Clare, Simon

AU - Brandt, Cordelia

AU - Mayho, Matthew

AU - Joffré, Enrique

AU - Sjöling, Åsa

AU - Turner, A. Keith

AU - Klena, John D.

AU - Kingsley, Robert A.

AU - Hill-Cawthorne, Grant A.

AU - Dougan, Gordon

AU - Pickard, Derek

N1 - Funding Information: This work was supported by The Wellcome Trust (G.D.) and Marie Bashir Institute and Sydney Medical School foundation (M.A. and G.A.H.). The work was also supported by an ESPRC Vaccine-Hub grant and the NIMR Cambridge BRC Antibiotic Resistance theme (G.D.) and the Swedish Research Council (Å.S.). R.A.K. was funded by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent project BBS/E/F/000PR10348. The funders had no role in study design, data collection and interpretation or the decision to submit the work for publication.

PY - 2021/6/10

Y1 - 2021/6/10

N2 - Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.

AB - Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.

KW - CFA/I

KW - ETEC

KW - H10407

KW - RNAseq

KW - TraDIS

KW - Water survival and colonization

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U2 - 10.1099/MGEN.0.000554

DO - 10.1099/MGEN.0.000554

M3 - Article

C2 - 34110281

AN - SCOPUS:85108123987

VL - 7

JO - Microbial Genomics

JF - Microbial Genomics

SN - 2057-5858

IS - 6

M1 - 000554

ER -

Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

Abstract

Keywords

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