G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C. Macaulay, Wilfried Haerty, Parveen Kumar, Yang I. Li, Tim Xiaoming Hu, Mabel J. Teng, Mubeen Goolam, Nathalie Saurat, Paul Coupland, Lesley M. Shirley, Miriam Smith, Niels Van Der Aa, Ruby Banerjee, Peter D. Ellis, Michael A. Quail, Harold P. Swerdlow, Magdalena Zernicka-Goetz, Frederick J. Livesey, Chris P. Ponting, Thierry Voet

Research output: Contribution to journalArticlepeer-review

522 Citations (Scopus)


The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.
Original languageEnglish
Pages (from-to)519-522
Number of pages4
JournalNature Methods
Issue number6
Early online date27 Apr 2015
Publication statusPublished - 1 Jun 2015

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