G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C. Macaulay; Wilfried Haerty; Parveen Kumar; Yang I. Li; Tim Xiaoming Hu; Mabel J. Teng; Mubeen Goolam; Nathalie Saurat; Paul Coupland; Lesley M. Shirley; Miriam Smith; Niels Van Der Aa; Ruby Banerjee; Peter D. Ellis; Michael A. Quail; Harold P. Swerdlow; Magdalena Zernicka-Goetz; Frederick J. Livesey; Chris P. Ponting; Thierry Voet

doi:10.1038/nmeth.3370

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C. Macaulay
, Wilfried Haerty
, Parveen Kumar
, Yang I. Li
, Tim Xiaoming Hu
, Mabel J. Teng
, Mubeen Goolam
, Nathalie Saurat
, Paul Coupland
, Lesley M. Shirley
, Miriam Smith
, Niels Van Der Aa
, Ruby Banerjee
, Peter D. Ellis
, Michael A. Quail
, Harold P. Swerdlow
, Magdalena Zernicka-Goetz
, Frederick J. Livesey
, Chris P. Ponting
, Thierry Voet

Research output: Contribution to journal › Article › peer-review

639 Citations (Scopus)

Abstract

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

Original language	English
Pages (from-to)	519-522
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	6
Early online date	27 Apr 2015
DOIs	https://doi.org/10.1038/nmeth.3370
Publication status	Published - 1 Jun 2015

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10.1038/nmeth.3370

http://www.nature.com/articles/nmeth.3370

Wilfried Haerty
- W.Haertyuea.acuk
- School of Biological Sciences - Senior Group Leader
- Norwich Institute for Healthy Aging - Member
Person: Research Centre Member, Academic, Teaching and Research
Iain Macaulay
- I.Macaulayuea.acuk
- School of Biological Sciences - Technical Development Group Leader
Person: Academic, Teaching and Research

Cite this

Macaulay, I. C., Haerty, W., Kumar, P., Li, Y. I., Hu, T. X., Teng, M. J., Goolam, M., Saurat, N., Coupland, P., Shirley, L. M., Smith, M., Van Der Aa, N., Banerjee, R., Ellis, P. D., Quail, M. A., Swerdlow, H. P., Zernicka-Goetz, M., Livesey, F. J., Ponting, C. P., & Voet, T. (2015). G&T-seq: parallel sequencing of single-cell genomes and transcriptomes. Nature Methods, 12(6), 519-522. https://doi.org/10.1038/nmeth.3370

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title = "G\&T-seq: parallel sequencing of single-cell genomes and transcriptomes",

abstract = "The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G\&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G\&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.",

author = "Macaulay, \{Iain C.\} and Wilfried Haerty and Parveen Kumar and Li, \{Yang I.\} and Hu, \{Tim Xiaoming\} and Teng, \{Mabel J.\} and Mubeen Goolam and Nathalie Saurat and Paul Coupland and Shirley, \{Lesley M.\} and Miriam Smith and \{Van Der Aa\}, Niels and Ruby Banerjee and Ellis, \{Peter D.\} and Quail, \{Michael A.\} and Swerdlow, \{Harold P.\} and Magdalena Zernicka-Goetz and Livesey, \{Frederick J.\} and Ponting, \{Chris P.\} and Thierry Voet",

year = "2015",

month = jun,

day = "1",

doi = "10.1038/nmeth.3370",

language = "English",

volume = "12",

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journal = "Nature Methods",

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Macaulay, IC , Haerty, W, Kumar, P, Li, YI, Hu, TX, Teng, MJ, Goolam, M, Saurat, N, Coupland, P, Shirley, LM, Smith, M, Van Der Aa, N, Banerjee, R, Ellis, PD, Quail, MA, Swerdlow, HP, Zernicka-Goetz, M, Livesey, FJ, Ponting, CP & Voet, T 2015, 'G&T-seq: parallel sequencing of single-cell genomes and transcriptomes', Nature Methods, vol. 12, no. 6, pp. 519-522. https://doi.org/10.1038/nmeth.3370

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T1 - G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

AU - Macaulay, Iain C.

AU - Haerty, Wilfried

AU - Kumar, Parveen

AU - Li, Yang I.

AU - Hu, Tim Xiaoming

AU - Teng, Mabel J.

AU - Goolam, Mubeen

AU - Saurat, Nathalie

AU - Coupland, Paul

AU - Shirley, Lesley M.

AU - Smith, Miriam

AU - Van Der Aa, Niels

AU - Banerjee, Ruby

AU - Ellis, Peter D.

AU - Quail, Michael A.

AU - Swerdlow, Harold P.

AU - Zernicka-Goetz, Magdalena

AU - Livesey, Frederick J.

AU - Ponting, Chris P.

AU - Voet, Thierry

PY - 2015/6/1

Y1 - 2015/6/1

N2 - The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

AB - The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

U2 - 10.1038/nmeth.3370

DO - 10.1038/nmeth.3370

M3 - Article

SN - 1548-7091

VL - 12

SP - 519

EP - 522

JO - Nature Methods

JF - Nature Methods

IS - 6

ER -

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Abstract

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Wilfried Haerty

Iain Macaulay

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