Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs

Abhijeet Deb Roy, Sabine Gruschow, Nickiwe Cairns, Rebecca J. M. Goss

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)


Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.
Original languageEnglish
Pages (from-to)12243-12245
Number of pages3
JournalJournal of the American Chemical Society
Issue number35
Publication statusPublished - 16 Aug 2010

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