Gene expression enabling synthetic diversification of natural products: Chemogenetic generation of pacidamycin analogs

Abhijeet Deb Roy; Sabine Gruschow; Nickiwe Cairns; Rebecca J. M. Goss

doi:10.1021/ja1060406

Gene expression enabling synthetic diversification of natural products: Chemogenetic generation of pacidamycin analogs

Abhijeet Deb Roy
, Sabine Gruschow
, Nickiwe Cairns
, Rebecca J. M. Goss

Research output: Contribution to journal › Article › peer-review

143 Citations (Scopus)

Abstract

Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.

Original language	English
Pages (from-to)	12243-12245
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	132
Issue number	35
DOIs	https://doi.org/10.1021/ja1060406
Publication status	Published - 16 Aug 2010

Access to Document

10.1021/ja1060406

http://dx.doi.org/10.1021/ja1060406

Cite this

@article{fc760f885b3f4f62a64318755dc483ff,

title = "Gene expression enabling synthetic diversification of natural products: Chemogenetic generation of pacidamycin analogs",

abstract = "Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.",

author = "Roy, \{Abhijeet Deb\} and Sabine Gruschow and Nickiwe Cairns and Goss, \{Rebecca J. M.\}",

year = "2010",

month = aug,

day = "16",

doi = "10.1021/ja1060406",

language = "English",

volume = "132",

pages = "12243--12245",

journal = "Journal of the American Chemical Society",

issn = "1520-5126",

publisher = "American Chemical Society",

number = "35",

}

TY - JOUR

T1 - Gene expression enabling synthetic diversification of natural products: Chemogenetic generation of pacidamycin analogs

AU - Roy, Abhijeet Deb

AU - Gruschow, Sabine

AU - Cairns, Nickiwe

AU - Goss, Rebecca J. M.

PY - 2010/8/16

Y1 - 2010/8/16

N2 - Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.

AB - Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.

U2 - 10.1021/ja1060406

DO - 10.1021/ja1060406

M3 - Article

SN - 1520-5126

VL - 132

SP - 12243

EP - 12245

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 35

ER -