Genetic evidence for natural product-mediated plant-plant allelopathy in rice (Oryza sativa)

Meimei Xu, Rita Galhano, Philipp Wiemann, Emilio Bueno, Mollie Tiernan, William Wu, Ill-Min Chung, Jonathan Gershenzon, Bettina Tudzynski, Ane Sesma, Reuben J. Peters

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

There is controversy as to whether specific natural products play a role in directly mediating antagonistic plant–plant interactions – that is, allelopathy. If proved to exist, such phenomena would hold considerable promise for agronomic improvement of staple food crops such as rice (Oryza sativa).


However, while substantiated by the presence of phytotoxic compounds at potentially relevant concentrations, demonstrating a direct role for specific natural products in allelopathy has been difficult because of the chemical complexity of root and plant litter exudates. This complexity can be bypassed via selective genetic manipulation to ablate production of putative allelopathic compounds, but such an approach previously has not been applied.


The rice diterpenoid momilactones provide an example of natural products for which correlative biochemical evidence has been obtained for a role in allelopathy. Here, we apply reverse genetics, using knock‐outs of the relevant diterpene synthases (copalyl diphosphate synthase 4 (OsCPS4) and kaurene synthase‐like 4 (OsKSL4)), to demonstrate that rice momilactones are involved in allelopathy, including suppressing growth of the widespread rice paddy weed, barnyard grass (Echinochloa crus‐galli).


Thus, our results not only provide novel genetic evidence for natural product‐mediated allelopathy, but also furnish a molecular target for breeding and metabolic engineering of this important crop plant.
Original languageEnglish
Pages (from-to)570-575
Number of pages6
JournalNew Phytologist
Volume193
Issue number3
Early online date12 Dec 2011
DOIs
Publication statusPublished - Feb 2012

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