TY - JOUR
T1 - Genome characterisation of an isoprene-degrading Alcaligenes sp. isolated from a tropical restored forest
AU - Uttarotai, Toungporn
AU - Sutheeworapong, Sawannee
AU - Crombie, Andrew T.
AU - Murrell, J. Colin
AU - Mhuantong, Wuttichai
AU - Noirungsee, Nuttapol
AU - Wangkarn, Sunanta
AU - Bovonsombut, Sakunnee
AU - McGenity, Terry J.
AU - Chitov, Thararat
N1 - Funding Information: This research was funded by Science Achievement Scholarship of Thailand (granted to T.U), Biodiversity-based Economy Development Office (Public Organisation) (BEDO) grant to T.C. and S.B (36/2562), Natural Environment Research Council (NERC) grant to T.J.M. (NE/J009555/1)), European Research Council (ERC) Advanced Grant (IsoMet 694578) to J.C.M. and partially supported by Chiang Mai University, Thailand and the University of Essex, UK.
PY - 2022/4
Y1 - 2022/4
N2 - Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
AB - Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
KW - Alcaligenes
KW - climate-active gas
KW - genome
KW - isoprene
KW - isoprene degradation
UR - http://www.scopus.com/inward/record.url?scp=85127980233&partnerID=8YFLogxK
U2 - 10.3390/biology11040519
DO - 10.3390/biology11040519
M3 - Article
VL - 11
JO - Biology
JF - Biology
SN - 2079-7737
IS - 4
M1 - 519
ER -
