Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b

Lisa Y. Stein; Sukhwan Yoon; Jeremy D. Semrau; Alan A. DiSpirito; Andrew Crombie; J. Colin Murrell; Stéphane Vuilleumier; Marina G. Kalyuzhnaya; Huub J. M. op den Camp; Françoise Bringel; D. Bruce; J.-F. Cheng; A. Copeland; Lynne Goodwin; Shunsheng Han; Loren Hauser; Mike S. M. Jetten; Aurélie Lajus; M. L. Land; A. Lapidus; S. Lucas; Claudine Médigue; S. Pitluck; Tanja Woyke; Ahmet Zeytun; Martin G. Klotz

doi:10.1128/JB.01144-10

Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b

Lisa Y. Stein, Sukhwan Yoon, Jeremy D. Semrau, Alan A. DiSpirito, Andrew Crombie, J. Colin Murrell, Stéphane Vuilleumier, Marina G. Kalyuzhnaya, Huub J. M. op den Camp, Françoise Bringel, D. Bruce, J.-F. Cheng, A. Copeland, Lynne Goodwin, Shunsheng Han, Loren Hauser, Mike S. M. Jetten, Aurélie Lajus, M. L. Land, A. LapidusS. Lucas, Claudine Médigue, S. Pitluck, Tanja Woyke, Ahmet Zeytun, Martin G. Klotz

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90 Citations (Scopus)

Abstract

Methylosinus trichosporium OB3b (for “oddball” strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales

Original language	English
Pages (from-to)	6497-6498
Number of pages	2
Journal	Journal of Bacteriology
Volume	192
Issue number	24
DOIs	https://doi.org/10.1128/JB.01144-10
Publication status	Published - Dec 2010

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10.1128/JB.01144-10

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Stein, L. Y., Yoon, S., Semrau, J. D., DiSpirito, A. A., Crombie, A., Murrell, J. C., Vuilleumier, S., Kalyuzhnaya, M. G., op den Camp, H. J. M., Bringel, F., Bruce, D., Cheng, J-F., Copeland, A., Goodwin, L., Han, S., Hauser, L., Jetten, M. S. M., Lajus, A., Land, M. L., ... Klotz, M. G. (2010). Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b. Journal of Bacteriology, 192(24), 6497-6498. https://doi.org/10.1128/JB.01144-10

@article{7c8f7f4dd0da49b29e1b15574cd10b2a,

title = "Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b",

abstract = "Methylosinus trichosporium OB3b (for “oddball” strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales",

author = "Stein, {Lisa Y.} and Sukhwan Yoon and Semrau, {Jeremy D.} and DiSpirito, {Alan A.} and Andrew Crombie and Murrell, {J. Colin} and St{\'e}phane Vuilleumier and Kalyuzhnaya, {Marina G.} and {op den Camp}, {Huub J. M.} and Fran{\c c}oise Bringel and D. Bruce and J.-F. Cheng and A. Copeland and Lynne Goodwin and Shunsheng Han and Loren Hauser and Jetten, {Mike S. M.} and Aur{\'e}lie Lajus and Land, {M. L.} and A. Lapidus and S. Lucas and Claudine M{\'e}digue and S. Pitluck and Tanja Woyke and Ahmet Zeytun and Klotz, {Martin G.}",

year = "2010",

month = dec,

doi = "10.1128/JB.01144-10",

language = "English",

volume = "192",

pages = "6497--6498",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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Stein, LY, Yoon, S, Semrau, JD, DiSpirito, AA, Crombie, A, Murrell, JC, Vuilleumier, S, Kalyuzhnaya, MG, op den Camp, HJM, Bringel, F, Bruce, D, Cheng, J-F, Copeland, A, Goodwin, L, Han, S, Hauser, L, Jetten, MSM, Lajus, A, Land, ML, Lapidus, A, Lucas, S, Médigue, C, Pitluck, S, Woyke, T, Zeytun, A & Klotz, MG 2010, 'Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b', Journal of Bacteriology, vol. 192, no. 24, pp. 6497-6498. https://doi.org/10.1128/JB.01144-10

TY - JOUR

T1 - Genome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b

AU - Stein, Lisa Y.

AU - Yoon, Sukhwan

AU - Semrau, Jeremy D.

AU - DiSpirito, Alan A.

AU - Crombie, Andrew

AU - Murrell, J. Colin

AU - Vuilleumier, Stéphane

AU - Kalyuzhnaya, Marina G.

AU - op den Camp, Huub J. M.

AU - Bringel, Françoise

AU - Bruce, D.

AU - Cheng, J.-F.

AU - Copeland, A.

AU - Goodwin, Lynne

AU - Han, Shunsheng

AU - Hauser, Loren

AU - Jetten, Mike S. M.

AU - Lajus, Aurélie

AU - Land, M. L.

AU - Lapidus, A.

AU - Lucas, S.

AU - Médigue, Claudine

AU - Pitluck, S.

AU - Woyke, Tanja

AU - Zeytun, Ahmet

AU - Klotz, Martin G.

PY - 2010/12

Y1 - 2010/12

N2 - Methylosinus trichosporium OB3b (for “oddball” strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales

AB - Methylosinus trichosporium OB3b (for “oddball” strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales

U2 - 10.1128/JB.01144-10

DO - 10.1128/JB.01144-10

M3 - Article

VL - 192

SP - 6497

EP - 6498

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 24

ER -