Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays

Jeremy Clark; Sandra Edwards; Andrew Feber; Penny Flohr; Megan John; Ian Giddings; Sue Crossland; Michael R Stratton; Richard Wooster; Colin Campbell; Colin S Cooper

doi:10.1038/sj.onc.1206247

Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays

Jeremy Clark, Sandra Edwards, Andrew Feber, Penny Flohr, Megan John, Ian Giddings, Sue Crossland, Michael R Stratton, Richard Wooster, Colin Campbell, Colin S Cooper

Research output: Contribution to journal › Article › peer-review

86 Citations (Scopus)

Abstract

We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete loss at 17q21.31 (500 kb spanning STAT3) and at 10q23.1 (50-350 kb spanning SFTPA2) in the PC3 cell line.

Original language	English
Pages (from-to)	1247-52
Number of pages	6
Journal	Oncogene
Volume	22
Issue number	8
DOIs	https://doi.org/10.1038/sj.onc.1206247
Publication status	Published - 27 Feb 2003

Keywords

Adenocarcinoma
Chromosome Mapping
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 17
DNA, Complementary
DNA, Neoplasm
Female
Gene Deletion
Gene Expression Profiling
Humans
Male
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
Prostatic Neoplasms
Tumor Cells, Cultured

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/sj.onc.1206247

Cite this

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title = "Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays",

abstract = "We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete loss at 17q21.31 (500 kb spanning STAT3) and at 10q23.1 (50-350 kb spanning SFTPA2) in the PC3 cell line.",

keywords = "Adenocarcinoma, Chromosome Mapping, Chromosomes, Human, Pair 10, Chromosomes, Human, Pair 17, DNA, Complementary, DNA, Neoplasm, Female, Gene Deletion, Gene Expression Profiling, Humans, Male, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Prostatic Neoplasms, Tumor Cells, Cultured",

author = "Jeremy Clark and Sandra Edwards and Andrew Feber and Penny Flohr and Megan John and Ian Giddings and Sue Crossland and Stratton, {Michael R} and Richard Wooster and Colin Campbell and Cooper, {Colin S}",

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T1 - Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays

AU - Clark, Jeremy

AU - Edwards, Sandra

AU - Feber, Andrew

AU - Flohr, Penny

AU - John, Megan

AU - Giddings, Ian

AU - Crossland, Sue

AU - Stratton, Michael R

AU - Wooster, Richard

AU - Campbell, Colin

AU - Cooper, Colin S

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AB - We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete loss at 17q21.31 (500 kb spanning STAT3) and at 10q23.1 (50-350 kb spanning SFTPA2) in the PC3 cell line.

KW - Adenocarcinoma

KW - Chromosome Mapping

KW - Chromosomes, Human, Pair 10

KW - Chromosomes, Human, Pair 17

KW - DNA, Complementary

KW - DNA, Neoplasm

KW - Female

KW - Gene Deletion

KW - Gene Expression Profiling

KW - Humans

KW - Male

KW - Nucleic Acid Hybridization

KW - Oligonucleotide Array Sequence Analysis

KW - Prostatic Neoplasms

KW - Tumor Cells, Cultured

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DO - 10.1038/sj.onc.1206247

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JO - Oncogene

JF - Oncogene

SN - 0950-9232

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