Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei

Matthew T. G. Holden, Richard W. Titball, Sharon J. Peacock, Ana M. Cerdeño-Tárraga, Timothy Atkins, Lisa C. Crossman, Tyrone Pitt, Carol Churcher, Karen Mungall, Stephen D. Bentley, Mohammed Sebaihia, Nicholas R. Thomson, Mathalie Bason, Ifor R. Beacham, Karen Brooks, Katherine A. Brown, Nat F. Brown, Greg L. Challis, Inna Cherevach, Tracy ChillingworthAnn Cronin, Ben Crossett, Paul Davis, David DeShazer, Theresa Feltwell, Audrey Fraser, Zahra Hance, Heidi Hauser, Simon Holroyd, Kay Jagels, Karen E. Keith, Mark Maddison, Sharon Moule, Claire Price, Michael A. Quail, Ester Rabbinowitsch, Kim Rutherford, Mandy Sanders, Mark Simmonds, Sirirurg Songsivilai, Kim Stevens, Sarinna Tumapa, Monkgol Vesaratchavest, Sally Whitehead, Corin Yeats, Bart G. Barrell, Petra C. F. Oyston, Julian Parkhill

Research output: Contribution to journalArticlepeer-review

633 Citations (Scopus)

Abstract

Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world and accounts for 20% of community-acquired septicaemias in northeastern Thailand where half of those affected die. Here we report the complete genome of B. pseudomallei, which is composed of two chromosomes of 4.07 megabase pairs and 3.17 megabase pairs, showing significant functional partitioning of genes between them. The large chromosome encodes many of the core functions associated with central metabolism and cell growth, whereas the small chromosome carries more accessory functions associated with adaptation and survival in different niches. Genomic comparisons with closely and more distantly related bacteria revealed a greater level of gene order conservation and a greater number of orthologous genes on the large chromosome, suggesting that the two replicons have distinct evolutionary origins. A striking feature of the genome was the presence of 16 genomic islands (GIs) that together made up 6.1% of the genome. Further analysis revealed these islands to be variably present in a collection of invasive and soil isolates but entirely absent from the clonally related organism B. mallei. We propose that variable horizontal gene acquisition by β. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species.

Original languageEnglish
Pages (from-to)14240-14245
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number39
Early online date17 Sep 2004
DOIs
Publication statusPublished - 28 Sep 2004

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